DNA-Methylation Signatures of Tobacco Smoking in a High Cardiovascular Risk Population: Modulation by the Mediterranean Diet
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comunitat-uji-handle2:10234/7038
comunitat-uji-handle3:10234/8634
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Title
DNA-Methylation Signatures of Tobacco Smoking in a High Cardiovascular Risk Population: Modulation by the Mediterranean DietAuthor (s)
Date
2023-02-18Publisher
MDPIISSN
1661-7827Bibliographic citation
Fernández-Carrión R, Sorlí JV, Asensio EM, Pascual EC, Portolés O, Alvarez-Sala A, Francès F, Ramírez-Sabio JB, Pérez-Fidalgo A, Villamil LV, et al. DNA-Methylation Signatures of Tobacco Smoking in a High Cardiovascular Risk Population: Modulation by the Mediterranean Diet. International Journal of Environmental Research and Public Health. 2023; 20(4):3635. https://doi.org/10.3390/ijerph20043635Type
info:eu-repo/semantics/articlePublisher version
https://www.mdpi.com/1660-4601/20/4/3635Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Biomarkers based on DNA methylation are relevant in the field of environmental health for precision health. Although tobacco smoking is one of the factors with a strong and consistent impact on DNA methylation, there ... [+]
Biomarkers based on DNA methylation are relevant in the field of environmental health for precision health. Although tobacco smoking is one of the factors with a strong and consistent impact on DNA methylation, there are very few studies analyzing its methylation signature in southern European populations and none examining its modulation by the Mediterranean diet at the epigenome-wide level. We examined blood methylation smoking signatures on the EPIC 850 K array in this population (n = 414 high cardiovascular risk subjects). Epigenome-wide methylation studies (EWASs) were performed analyzing differential methylation CpG sites by smoking status (never, former, and current smokers) and the modulation by adherence to a Mediterranean diet score was explored. Gene-set enrichment analysis was performed for biological and functional interpretation. The predictive value of the top differentially methylated CpGs was analyzed using receiver operative curves. We characterized the DNA methylation signature of smoking in this Mediterranean population by identifying 46 differentially methylated CpGs at the EWAS level in the whole population. The strongest association was observed at the cg21566642 (p = 2.2 × 10−32) in the 2q37.1 region. We also detected other CpGs that have been consistently reported in prior research and discovered some novel differentially methylated CpG sites in subgroup analyses. In addition, we found distinct methylation profiles based on the adherence to the Mediterranean diet. Particularly, we obtained a significant interaction between smoking and diet modulating the cg5575921 methylation in the AHRR gene. In conclusion, we have characterized biomarkers of the methylation signature of tobacco smoking in this population, and suggest that the Mediterranean diet can increase methylation of certain hypomethylated sites. [-]
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International Journal of Environmental Research and Public Health. 2023; 20(4)Funder Name
Competitividad-Fondo Europeo de Desarrollo Regional | Generalitat Valenciana | Instituto de Salud Carlos III | European Regional Development Fund | Consejería de Salud y Familias, Junta de Andalucía | Agencia Estatal de Investigación | Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital, Generalitat Valenciana
Project code
CIBER 06/03, PI06/1326, PI13/00728, PI16/00366, PI19/00781, PID2019-108858RB-I00, SAF2016–80532-R | APOSTD/2020/164, PROMETEO 17/2017, PROMETEO/2021/021
Rights
© 2023 by the authors.
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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- LSI_Articles [362]