Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological Characterization
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https://doi.org/10.3791/65635 |
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Títol
Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological CharacterizationAutoria
Data de publicació
2023-11-17Editor
MyJove CorporationISSN
1940-087XCita bibliogràfica
Giner Moreno, E., Quereda-Flores, F., Hernández Chinchilla, J.A., Amigo Moreno, R., Perez Ruiz, M.D., Carcelén López, A., Rocher Benlloch, J., Molió Sanchís, P., Casares Calzada, A., Marqués-Torrejón, M.Á., MartínezCadenas, C., Ruiz Cerdá, J.L., Loras Monfort, A., Ramos Soler, D. Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological Characterization. J. Vis. Exp. (201), e65635, doi:10.3791/65635 (2023).Tipus de document
info:eu-repo/semantics/articleVersió
info:eu-repo/semantics/acceptedVersionParaules clau / Matèries
Resum
Acquiring fresh and well-characterized tumor tissue samples is critical for conducting
high-quality "omics" studies. However, it can be particularly challenging in the context
of prostate cancer (PC) due to the ... [+]
Acquiring fresh and well-characterized tumor tissue samples is critical for conducting
high-quality "omics" studies. However, it can be particularly challenging in the context
of prostate cancer (PC) due to the unique nature of this organ and the high
heterogeneity associated with this tumor. On the other hand, histopathologically
characterizing samples before their storage without causing significant tissue
alterations is also an intriguing challenge. In this context, we present a new method
for acquiring, mapping, characterizing, and micro-dissecting resected prostate tissue
based on anatomopathological criteria.
Unlike previously published protocols, this method reduces the time required
for histopathological analysis of the prostate specimen without compromising its
structure, which is crucial for assessing surgical margins. Furthermore, it enables
the delineation and micro-macro dissection of fresh prostate tissue samples, with a
focus on histological tumor areas defined by pathological criteria such as Gleason
score, precursor lesions (high-grade prostatic intraepithelial neoplasia - PIN), and
inflammatory lesions (prostatitis). These samples are then stored in a Biobank for
subsequent research analyses. [-]
Entitat finançadora
"Margarita Salas" postdoctoral contract | 'Maria Zambrano' research contract | European Union-Next Generation EU
Codi del projecte o subvenció
21-076 | MAZ/2021/03 UP2021-021
Drets d'accés
Copyright © 2023 JoVE Journal of Visualized Experiments jove.com
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