Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion Pressure
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Títol
Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion PressureAutoria
Data de publicació
2024-01-31Editor
American Chemical SocietyISSN
1549-9596; 1549-960XCita bibliogràfica
Aguilella-Arzo, M., Hoogerheide, D. P., Doucet, M., Wang, H., & Aguilella, V. M. (2024). Charged Biological Membranes Repel Large Neutral Molecules by Surface Dielectrophoresis and Counterion Pressure. Journal of the American Chemical Society, 146(4), 2701-2710.Tipus de document
info:eu-repo/semantics/articleVersió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
Macromolecular crowding is the usual condition of cells. The implications of the crowded cellular environment for protein stability and folding, protein–protein interactions, and intracellular transport drive a growing ... [+]
Macromolecular crowding is the usual condition of cells. The implications of the crowded cellular environment for protein stability and folding, protein–protein interactions, and intracellular transport drive a growing interest in quantifying the effects of crowding. While the properties of crowded solutions have been extensively studied, less attention has been paid to the interaction of crowders with the cellular boundaries, i.e., membranes. However, membranes are key components of cells and most subcellular organelles, playing a central role in regulating protein channel and receptor functions by recruiting and binding charged and neutral solutes. While membrane interactions with charged solutes are dominated by electrostatic forces, here we show that significant charge-induced forces also exist between membranes and neutral solutes. Using neutron reflectometry measurements and molecular dynamics simulations of poly(ethylene glycol) (PEG) polymers of different molecular weights near charged and neutral membranes, we demonstrate the roles of surface dielectrophoresis and counterion pressure in repelling PEG from charged membrane surfaces. The resulting depletion zone is expected to have consequences for drug design and delivery, the activity of proteins near membrane surfaces, and the transport of small molecules along the membrane surface. [-]
Publicat a
J. Am. Chem. Soc. 2024, 146, 4, 2701–2710Dades relacionades
https://pubs.acs.org/doi/10.1021/jacs.3c12348?goto=supporting-info&ref=pdfEntitat finançadora
National Institute of Standards and Technology | National Science Foundation | Scientific User Facilities Division | Ministerio de Ciencia, Innovación y Universidades | European Union | Universitat Jaume I
Codi del projecte o subvenció
DMR-2010792 | AC05-00OR22725. M.A-A. | AC05-00OR22725. V.M.A. | MCIN/AEI/10.13039/501100011033/FEDER | PID2022-142795 NB-I00 | UJI-B2022-42
Drets d'accés
Copyright © 2024 The Authors. Published by American Chemical Society.
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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