Gold nanoparticle-based two-photon fluorescent nanoprobe for monitoring intracellular nitric oxide levels
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Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
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INVESTIGACIONMetadatos
Título
Gold nanoparticle-based two-photon fluorescent nanoprobe for monitoring intracellular nitric oxide levelsAutoría
Fecha de publicación
2023-03-08Editor
Royal Society of ChemistryCita bibliográfica
Del Valle, C. A., Thomas, P., Galindo, F., Muñoz, M. P., & Marín, M. J. (2023). Gold nanoparticle-based two-photon fluorescent nanoprobe for monitoring intracellular nitric oxide levels. Journal of Materials Chemistry B, 11(15), 3387-3396.Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Nitric oxide (NO) plays an important role in the regulation of the immune, cardiovascular and nervous systems. Consequently, being able to monitor and quantify intracellular NO levels would provide a greater underst ... [+]
Nitric oxide (NO) plays an important role in the regulation of the immune, cardiovascular and nervous systems. Consequently, being able to monitor and quantify intracellular NO levels would provide a greater understanding of the implications of this molecule in the different biological processes, including, for example, in cancer. Here, we report a broadly applicable two-photon excitable fluorescent nanoprobe able to detect and potentially quantify NO levels in an extensive range of cellular environments. The nanoprobe consists of a thiolated photoinduced electron transfer-based two=photon fluorescent probe attached onto the surface of 2.4 ± 0.7 nm gold nanoparticles (DANPY-NO@AuNPs). The nanoprobe, which can be synthesised in a reproducible manner and exhibits great stability when stored at room temperature, is able to selectively detect NO in solution, with a dynamic range up to 150 μM, and at pH values of biological relevance. DANPY-NO@AuNPs were able to selectively detect endogenous NO in RAW264.7γ NO− macrophages and THP-1 human leukemic cells; and endogenous and exogenous NO in endothelial cells. The nanoprobe accumulated in the acidic organelles of the tested cell lines showing negligible toxicity. Importantly, DANPY-NO@AuNPs showed potential to quantify intracellular NO concentrations in MDA-MB-231 breast cancer cells. The biological evaluation of the nanoprobe was undertaken using confocal laser scanning (images and intracellular emission spectra) and multiphoton microscopies, and flow cytometry. Based on their excellent sensitivity and stability, and outstanding versatility, DANPY-NO@AuNPs can be applied for the spatiotemporal monitoring of in vitro and in vivo NO levels. [-]
Publicado en
J. Mater. Chem. B, 2023, 11, 3387Entidad financiadora
Faculty of Sciences and School of Chemistry at the University of East Anglia | Mr and Mrs Whittaker oncology fellowship for financial support | EPSRC
Código del proyecto o subvención
EP/S017909/1
Derechos de acceso
This journal is © The Royal Society of Chemistry 2023
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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