Triplin: Functional Probing of Its Structure and the Dynamics of the Voltage-Gating Process
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Otros documentos de la autoría: Aguilella, Vicente; Colombini, Marco; Barnes, Kevin; Chang, Kai-Ti; Younis, Muhsin
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comunitat-uji-handle2:10234/2507
comunitat-uji-handle3:10234/6973
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Título
Triplin: Functional Probing of Its Structure and the Dynamics of the Voltage-Gating ProcessFecha de publicación
2022-11-22Editor
MDPICita bibliográfica
Colombini, M.; Barnes, K.; Chang, K.-T.; Younis, M.H.; Aguilella, V.M. Triplin: Functional Probing of Its Structure and the Dynamics of the Voltage-Gating Process. Int. J. Mol. Sci. 2022, 23, 13765. https://doi.org/ 10.3390/ijms232213765Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.mdpi.com/1422-0067/23/22/13765Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Gram-negative bacteria have a large variety of channel-forming proteins in their outer
membrane, generally referred to as porins. Some display weak voltage dependence. A similar trimeric
channel former, named Triplin, ... [+]
Gram-negative bacteria have a large variety of channel-forming proteins in their outer
membrane, generally referred to as porins. Some display weak voltage dependence. A similar trimeric
channel former, named Triplin, displays very steep voltage dependence, rivaling that responsible
for the electrical excitability of mammals, and high inter-subunit cooperativity. We report detailed
insights into the molecular basis for these very unusual properties explored at the single-molecule
level. By using chemical modification to reduce the charge on the voltage sensors, they were shown
to be positively charged structures. Trypsin cleavage of the sensor eliminates voltage gating by
cleaving the sensor. From asymmetrical addition of these reagents, the positively charged voltage
sensors translocate across the membrane and are, thus, responsible energetically for the steep voltage
dependence. A mechanism underlying the cooperativity was also identified. Theoretical calculations
indicate that the charge on the voltage sensor can explain the rectification of the current flowing
through the open pores if it is located near the pore mouth in the open state. All results support the
hypothesis that one of the three subunits is oriented in a direction opposite to that of the other two.
These properties make Triplin perhaps the most complex pore-forming molecular machine described
to date. [-]
Publicado en
Int. J. Mol. Sci. 2022, 23(22), 13765Entidad financiadora
National Science Foundation | Ministerio de Ciencia, Innovación y Universidades (Spain)
Código del proyecto o subvención
MCB-1023008 | MCIN/AEI/10.13039/501100011033 (Project 2019-108434GB-I00)
Derechos de acceso
© 2022 by the authors
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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