Multimodular Bio-Inspired Organized Structures Guiding Long-Distance Axonal Regeneration
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Other documents of the author: Rodríguez Doblado, Laura; Martínez Ramos, Cristina; Monleón Pradas, Manuel
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Title
Multimodular Bio-Inspired Organized Structures Guiding Long-Distance Axonal RegenerationDate
2022-09Publisher
MDPIISSN
2227-9059Bibliographic citation
Rodríguez Doblado, L.; Martínez-Ramos, C.; Monleón Pradas, M. Multimodular Bio-Inspired Organized Structures Guiding Long-Distance Axonal Regeneration. Biomedicines 2022, 10, 2228. https://doi.org/10.3390/biomedicines10092228Type
info:eu-repo/semantics/articlePublisher version
https://www.mdpi.com/2227-9059/10/9/2228Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Axonal bundles or axonal tracts have an aligned and unidirectional architecture present in many neural structures with different lengths. When peripheral nerve injury (PNI), spinal cord injury (SCI), traumatic brain ... [+]
Axonal bundles or axonal tracts have an aligned and unidirectional architecture present in many neural structures with different lengths. When peripheral nerve injury (PNI), spinal cord injury (SCI), traumatic brain injury (TBI), or neurodegenerative disease occur, the intricate architecture undergoes alterations leading to growth inhibition and loss of guidance through large distance. In order to overcome the limitations of long-distance axonal regeneration, here we combine a poly-L-lactide acid (PLA) fiber bundle in the common lumen of a sequence of hyaluronic acid (HA) conduits or modules and pre-cultured Schwann cells (SC) as cells supportive of axon extension. This multimodular preseeded conduit is then used to induce axon growth from a dorsal root ganglion (DRG) explant placed at one of its ends and left for 21 days to follow axon outgrowth. The multimodular conduit proved effective in promoting directed axon growth, and the results may thus be of interest for the regeneration of long tissue defects in the nervous system. Furthermore, the hybrid structure grown within the HA modules consisting in the PLA fibers and the SC can be extracted from the conduit and cultured independently. This “neural cord” proved to be viable outside its scaffold and opens the door to the generation of ex vivo living nerve in vitro for transplantation. [-]
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Biomedicines 2022, vol. 10, no 9Funder Name
Ministerio de Educación, Cultura y Deporte | Ministerio de Economía y Competitividad | Ministerio de Ciencia, Innovación y Universidades
Funder ID
http://dx.doi.org/10.13039/501100003176 | http://dx.doi.org/10.13039/501100011033 | ttp://dx.doi.org/10.13039/501100003329
Project code
FPU15/04975 | MINECO//DPI2015-72863-EXP | MICIU/ICTI2017-2020/RTI2018-095872-B-C22
Project title or grant
Neurocables Modulares: multiplicando conexiones neurales | Nuevo dispositivo bioactivo para la regeneración de lesiones de la medula espinal
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info:eu-repo/semantics/openAccess
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