Identification of very long-chain (>C24) fatty acid methyl esters using gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with atmospheric pressure chemical ionization source
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Other documents of the author: Garlito Molina, Borja; Portoles, Tania; Niessen, Wilfried; Navarro, Juan C.; Hontoria, Francisco; Monroig, Oscar; Varó, Inmaculada; Serrano, Roque
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Show full item recordcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/33596
comunitat-uji-handle3:10234/33597
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INVESTIGACIONMetadata
Title
Identification of very long-chain (>C24) fatty acid methyl esters using gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with atmospheric pressure chemical ionization sourceAuthor (s)
Date
2019-03-21Publisher
ElsevierISSN
0003-2670Bibliographic citation
Garlito B., Portolés T., Niessen W.M.A., Navarro J.C., Hontoria F., Monroig Ó., Varó I., Serrano R. Identification of very long-chain (>C24) fatty acid methyl esters using gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with atmospheric pressure chemical ionization source. Anal. Chim. Acta. 2019;1051:103–109. doi: 10.1016/j.aca.2018.11.001.Type
info:eu-repo/semantics/articlePublisher version
https://www.sciencedirect.com/science/article/pii/S000326701831328XVersion
info:eu-repo/semantics/acceptedVersionSubject
Abstract
Gas chromatography coupled to a quadrupole time-of-flight mass analyzer (QTOF) with an atmospheric pressure chemical ionization (APCI) source has been tested to study the ionization and mass spectrometric behavior of ... [+]
Gas chromatography coupled to a quadrupole time-of-flight mass analyzer (QTOF) with an atmospheric pressure chemical ionization (APCI) source has been tested to study the ionization and mass spectrometric behavior of long-chain and very long-chain polyunsaturated fatty acids (LC-PUFAs, C18–24; VLC-PUFAs, >C24). The protonated molecule ([M+H]+), measured at accurate mass, became the base peak of the spectrum for all the studied compounds and was promoted by the addition of water into the source. This fact overcame the existing difficulties for the identification of VLC-PUFAs when using an electron ionization source (EI). The extensive fragmentation of PUFAs in this source is the main drawback due to the fact that since reference standards are not commercially available, final identification relies on retention time estimation. The application of GC−APCI-QTOF to the screening of lipid extracts from the eyes of different fish species added confidence to the identification of several VLC-PUFAs. Further investigation of ion ratios allowed to predict the position of key double bonds enabling the classification of VLC-PUFAs as ω3 or ω6 compounds. VLC-PUFAs spectra found in biological samples were compared to those obtained from corresponding peaks found in heterologous expression experiments of fish's Elovl4. [-]
Is part of
Analytica Chimica Acta, 2019, vol. 1051Funder Name
Ministerio de Economía y Competitividad (España) | Research Unit of Marine Ecotoxicology
Funder ID
http://dx.doi.org/10.13039/501100003329
Project code
MINECO/ICTI2013-2016/AGL2013-40986-R | MINECO/ICTI2013-2016/IJCI-2014-20588 | IATS-CSIC/IUPA-UJI
Project title or grant
Ácidos grasos poliinsaturados de cadena muy larga en peces: biosíntesis e implicaciones durante fases tempranas de desarrollo en especies cultivadas
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info:eu-repo/semantics/openAccess
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- IUPA_Articles [309]