Identification of very long-chain (>C24) fatty acid methyl esters using gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with atmospheric pressure chemical ionization source
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Metadades
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INVESTIGACIONMetadades
Títol
Identification of very long-chain (>C24) fatty acid methyl esters using gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with atmospheric pressure chemical ionization sourceAutoria
Data de publicació
2019-03-21Editor
ElsevierISSN
0003-2670Cita bibliogràfica
Garlito B., Portolés T., Niessen W.M.A., Navarro J.C., Hontoria F., Monroig Ó., Varó I., Serrano R. Identification of very long-chain (>C24) fatty acid methyl esters using gas chromatography coupled to quadrupole/time-of-flight mass spectrometry with atmospheric pressure chemical ionization source. Anal. Chim. Acta. 2019;1051:103–109. doi: 10.1016/j.aca.2018.11.001.Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
https://www.sciencedirect.com/science/article/pii/S000326701831328XVersió
info:eu-repo/semantics/acceptedVersionParaules clau / Matèries
Resum
Gas chromatography coupled to a quadrupole time-of-flight mass analyzer (QTOF) with an atmospheric pressure chemical ionization (APCI) source has been tested to study the ionization and mass spectrometric behavior of ... [+]
Gas chromatography coupled to a quadrupole time-of-flight mass analyzer (QTOF) with an atmospheric pressure chemical ionization (APCI) source has been tested to study the ionization and mass spectrometric behavior of long-chain and very long-chain polyunsaturated fatty acids (LC-PUFAs, C18–24; VLC-PUFAs, >C24). The protonated molecule ([M+H]+), measured at accurate mass, became the base peak of the spectrum for all the studied compounds and was promoted by the addition of water into the source. This fact overcame the existing difficulties for the identification of VLC-PUFAs when using an electron ionization source (EI). The extensive fragmentation of PUFAs in this source is the main drawback due to the fact that since reference standards are not commercially available, final identification relies on retention time estimation. The application of GC−APCI-QTOF to the screening of lipid extracts from the eyes of different fish species added confidence to the identification of several VLC-PUFAs. Further investigation of ion ratios allowed to predict the position of key double bonds enabling the classification of VLC-PUFAs as ω3 or ω6 compounds. VLC-PUFAs spectra found in biological samples were compared to those obtained from corresponding peaks found in heterologous expression experiments of fish's Elovl4. [-]
Publicat a
Analytica Chimica Acta, 2019, vol. 1051Entitat finançadora
Ministerio de Economía y Competitividad (España) | Research Unit of Marine Ecotoxicology
Identificador de l'entitat finançadora
http://dx.doi.org/10.13039/501100003329
Codi del projecte o subvenció
MINECO/ICTI2013-2016/AGL2013-40986-R | MINECO/ICTI2013-2016/IJCI-2014-20588 | IATS-CSIC/IUPA-UJI
Títol del projecte o subvenció
Ácidos grasos poliinsaturados de cadena muy larga en peces: biosíntesis e implicaciones durante fases tempranas de desarrollo en especies cultivadas
Drets d'accés
info:eu-repo/semantics/openAccess
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- IUPA_Articles [309]