Deciphering of benzothiadiazole (BTH)-induced response of tomato (Solanum lycopersicum L.) and its effect on early response to virus infection through the multi-omics approach
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Other documents of the author: Frąckowiak, Patryk Marek; Wrzesińska-Krupa, Barbara; Wieczorek, Przemysław; Sánchez-Bel, Paloma; Kunz, Laura; Dittmann, Antje; Obrepalska-Steplowska, Aleksandra
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Show full item recordcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
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Title
Deciphering of benzothiadiazole (BTH)-induced response of tomato (Solanum lycopersicum L.) and its effect on early response to virus infection through the multi-omics approachAuthor (s)
Date
2022-08-22Publisher
Springer NatureISSN
0032-079X; 1573-5036Bibliographic citation
Frąckowiak, P., Wrzesińska, B., Wieczorek, P. et al. Deciphering of benzothiadiazole (BTH)-induced response of tomato (Solanum lycopersicum L.) and its effect on early response to virus infection through the multi-omics approach. Plant Soil (2022).Type
info:eu-repo/semantics/articleVersion
info:eu-repo/semantics/publishedVersionSubject
Abstract
Background and aims:
One of the preventive methods used to limit the losses caused by viruses is the application of synthetic immunity inducers, such as benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester ... [+]
Background and aims:
One of the preventive methods used to limit the losses caused by viruses is the application of synthetic immunity inducers, such as benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH). This study aimed at explaining how the BTH treatment affects the defence and developmental processes in tomato plants (Solanum lycopersicum L.) as well as plant response to virus infection.
Method:
The comparative multi-omics analyses concerning tomato plants treated with BTH were performed, including transcriptomics (RNA-Seq), proteomics (Liquid Chromatography-Mass Spectrometry), and metabolomics (targeted hormonal analysis). To confirm the priming effect of BTH on tomato resistance, the plants were infected with tomato mosaic virus (ToMV) seven days post-BTH treatment.
Results:
The combined functional analysis indicated the high impact of BTH on the plant's developmental processes and activation of the immune response early after the treatment. In the presented experimental model, the increased level of WRKY TRANSCRIPTION FACTORS, ARGONAUTE 2A, thiamine and glutathione metabolism, cell wall reorganization, and detoxification processes, as well as accumulation of three phytohormones: abscisic acid, jasmonic acid-isoleucine (JA-Ile), and indole-3-carboxylic acid (I3CA), were observed upon BTH application.
Conclusion:
The immune response activated by BTH was related to increased expression of genes associated with the cellular detoxification process, systemic acquired resistance, and induced systemic resistance as well as post-transcriptional gene silencing. Increased levels of I3CA and JA-Ile might explain the BTH's effectiveness in the induction of the plant defence against a broad spectrum of pathogens. For the first time, the BTH involvement in the induction of the thiamine metabolism was revealed in tomatoes. [-]
Is part of
Plant and Soil (2022)Funder Name
National Science Centre (Poland) | European Union | Ministry of Education and Science in Poland
Project code
UMO-2015/17/B/NZ9/01676 | EPIC-XS 823839
Project title or grant
project OPUS | Horizon 2020
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info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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