A highly photostable and versatile two-photon fluorescent probe for the detection of a wide range of intracellular nitric oxide concentrations in macrophages and endothelial cells
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Otros documentos de la autoría: Arnau del Valle, Carla; Williams, Lewis; Thomas, Paul; Johnson, Robert T.; Raveenthiraraj, Sathuwarman; Warren, Derek; Sobolewski, Anastasia; Munoz, Maria Paz; Galindo, Francisco; Marin, Maria Jose
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
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INVESTIGACIONMetadatos
Título
A highly photostable and versatile two-photon fluorescent probe for the detection of a wide range of intracellular nitric oxide concentrations in macrophages and endothelial cellsAutoría
Fecha de publicación
2022-07-15Editor
ElsevierISSN
1011-1344; 1873-2682Cita bibliográfica
Del Valle, C. A., Williams, L., Thomas, P., Johnson, R., Raveenthiraraj, S., Warren, D., ... & Marín, M. J. (2022). A highly photostable and versatile two-photon fluorescent probe for the detection of a wide range of intracellular nitric oxide concentrations in macrophages and endothelial cells. Journal of Photochemistry and Photobiology B: Biology, 234, 112512.Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Nitric oxide (NO) is involved in many biological processes affecting the cardiovascular, nervous and immune systems. Intracellular NO can be monitored using fluorescent probes in combination with fluorescence imaging ... [+]
Nitric oxide (NO) is involved in many biological processes affecting the cardiovascular, nervous and immune systems. Intracellular NO can be monitored using fluorescent probes in combination with fluorescence imaging techniques. Most of the currently available NO fluorescent molecular probes are excited via one-photon excitation using UV or Vis light, which results in poor penetration and high photodamage to living tissues. Here, we report a two-photon fluorescent molecular probe, DANPY-NO, able to detect NO in live cells. The probe consists of an o-phenylenediamine linked to a naphthalimide core; and operates via photoinduced electron transfer. DANPY-NO exhibits good sensitivity (LOD of 77.8 nM) and high selectivity towards NO, and is stable over a broad range of pHs. The probe targeted acidic organelles within macrophages and endothelial cells, and demonstrated enhanced photostability over a commercially available NO probe. DANPY-NO was used to selectively detect endogenous NO in RAW264.7ϒ NO− macrophages, THP-1 human leukemic cells, primary mouse (bone marrow-derived) macrophages and endothelial cells. The probe was also able to detect exogenous NO in endothelial cells and distinguish between increasing concentrations of NO. The NO detection was evidenced using confocal laser scanning and two-photon microscopies, and flow cytometry. Further evidence was obtained by recording the changes in the intracellular fluorescence emission spectrum of the probe. Importantly, the probe displayed negligible toxicity to the analysed biological samples. The excellent sensitivity, selectivity, stability and versatility of DANPY-NO confirm its potential for in vitro and in vivo imaging of NO. [-]
Publicado en
Journal of Photochemistry & Photobiology, B: Biology 234 (2022) 112512Datos relacionados
https://ars.els-cdn.com/content/image/1-s2.0-S1011134422001269-mmc1.pdfEntidad financiadora
Engineering and Physical Sciences Research Council (EPSRC)
Código del proyecto o subvención
EP/S017909/1
Derechos de acceso
info:eu-repo/semantics/openAccess
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