Development of a Polymeric Film Entrapping Rose Bengal and Iodide Anion for the Light-Induced Generation and Release of Bactericidal Hydrogen Peroxide
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Other documents of the author: López Fernández, Ana María; Moisescu, Evelina Elena; de Llanos Frutos, Rosa; Galindo, Francisco
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comunitat-uji-handle2:10234/7053
comunitat-uji-handle3:10234/8639
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Title
Development of a Polymeric Film Entrapping Rose Bengal and Iodide Anion for the Light-Induced Generation and Release of Bactericidal Hydrogen PeroxideAuthor (s)
Date
2022-09-05Publisher
MDPIBibliographic citation
López-Fernández, A.M.; Moisescu, E.E.; de Llanos, R.; Galindo, F. Development of a Polymeric Film Entrapping Rose Bengal and Iodide Anion for the Light-Induced Generation and Release of Bactericidal Hydrogen Peroxide. Int. J. Mol. Sci. 2022, 23, 10162. https://doi.org/10.3390/ ijms231710162Type
info:eu-repo/semantics/articlePublisher version
https://www.mdpi.com/1422-0067/23/17/10162Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
A series of poly(2-hydroxyethyl methacrylate) (PHEMA) thin films entrapping photosensitizer Rose Bengal (RB) and tetrabutylammonium iodide (TBAI) have been synthetized. The
materials have been characterized by means ... [+]
A series of poly(2-hydroxyethyl methacrylate) (PHEMA) thin films entrapping photosensitizer Rose Bengal (RB) and tetrabutylammonium iodide (TBAI) have been synthetized. The
materials have been characterized by means of Thermogravimetric Analysis (TGA), Attenuated
Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) and UV-vis Absorption spectroscopy. Irradiation of the materials with white light led to the generation of several bactericidal
species, including singlet oxygen (1O2
), triiodide anion (I3
−) and hydrogen peroxide (H2O2
). 1O2
production was demonstrated spectroscopically by reaction with the chemical trap 2,20
-(anthracene9,10-diylbis(methylene))dimalonic acid (ABDA). In addition, the reaction of iodide anion with 1O2
yielded I3
− inside the polymeric matrix. This reaction is accompanied by the formation of H2O2
,
which diffuses out the polymeric matrix. Generation of both I3
− and H2O2 was demonstrated spectroscopically (directly in the case of triiodide by the absorption at 360 nm and indirectly for H2O2
using the xylenol orange test). A series of photodynamic inactivation assays were conducted with
the synthesized polymers against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa.
Complete eradication (7 log10 CFU/mL) of both bacteria occurred after only 5 min of white light
irradiation (400–700 nm; total energy dose 24 J/cm2
) of the polymer containing both RB and TBAI.
The control polymer without embedded iodide (only RB) showed only marginal reductions of ca. 0.5
log10 CFU/mL. The main novelty of the present investigation is the generation of three bactericidal
species (1O2
, I3
− and H2O2
) at the same time using a single polymeric material containing all the
elements needed to produce such a bactericidal cocktail, although the most relevant antimicrobial
activity is shown by H2O2
. This experimental approach avoids multistep protocols involving a final
step of addition of I−, as described previously for other assays in solution. [-]
Is part of
International Journal of Molecular Sciences. 2022, 23, 10162.Funder Name
Universitat Jaume I | Ministerio de Educación y Formación Profesional
Project code
UJI-B2021–51 | UJIA2020–15 | BG-P18/00062
Rights
© 2022 by the authors
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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- QUIO_Articles [701]