Proteomic evaluation of human osteoblast responses to titanium implants over time
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Proteomic evaluation of human osteoblast responses to titanium implants over timeAutoria
Data de publicació
2022-08-30Editor
Wiley; Wiley PeriodicalsISSN
1549-3296; 1552-4965Cita bibliogràfica
Romero-Gavilán F, Cerqueira A, García-Arnáez I, et al. Proteomic evaluation of human osteoblast responses to titanium implants over time. J Biomed Mater Res. 2022;1‐15. doi:10.1002/jbm.a.37444Tipus de document
info:eu-repo/semantics/articleVersió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
Titanium is widely used in bone prostheses due to its excellent biocompatibility and osseointegration capacity. To understand the effect of sandblasted acid-etched (SAE) Ti implants on the biological responses of human ... [+]
Titanium is widely used in bone prostheses due to its excellent biocompatibility and osseointegration capacity. To understand the effect of sandblasted acid-etched (SAE) Ti implants on the biological responses of human osteoblast (HOb), their proteomic profiles were analyzed using nLC-MS/MS. The cells were cultured with the implant materials, and 2544 distinct proteins were detected in samples taken after 1, 3, and 7 days. Comparative analyses of proteomic data were performed using Perseus software. The expression of proteins related to EIF2, mTOR, insulin-secretion and IGF pathways showed marked differences in cells grown with SAE-Ti in comparison with cells cultured without Ti. Moreover, the proteomic profiles obtained with SAE-Ti were compared over time. The affected proteins were related to adhesion, immunity, oxidative stress, coagulation, angiogenesis, osteogenesis, and extracellular matrix formation functions. The proliferation, mineralization and osteogenic gene expression in HObs cultured with SAE-Ti were characterized in vitro. The results showed that the osteoblasts exposed to this material increase their mineralization rate and expression of COLI, RUNX2, SP7, CTNNB1, CAD13, IGF2, MAPK2, and mTOR. Overall, the observed proteomic profiles can explain the SAE-Ti osteogenic properties, widening our knowledge of key signaling pathways taking part in the early stages of the osseointegration process in this type of implantations. [-]
Publicat a
J Biomed Mater Res. 2022;1–15Dades relacionades
jbma37444-sup-0001-TableS1.docx /// jbma37444-sup-0002-TableS2.docx /// jbma37444-sup-0003-TableS3.docxEntitat finançadora
Euskal Herriko Unibertsitatea | Generalitat Valenciana | Ministerio de Ciencia e Innovación | Universitat Jaume I
Codi del projecte o subvenció
GIU18/189 | APOSTD/2020/036 | PROMETEO/2020/069 | PID2020-113092RB-C21 | UJI-B2021-25
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info:eu-repo/semantics/openAccess
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© 2022 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC.