Characterization of microglial response in the female 3xTgAD Model
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Characterization of microglial response in the female 3xTgAD ModelAutoria
Tutor/Supervisor; Universitat.Departament
Ros Bernal, Francisco de Asís; Universitat Jaume I. Unitat Predepartamental de MedicinaData de publicació
2021-09-07Editor
Universitat Jaume IResum
Alzheimer's disease (AD) involves severe impairment of cognitive and executive functions and
represents 60-70% of all cases of dementia. Neuropathologically, it is characterized by the
deposition of beta-amyloid ... [+]
Alzheimer's disease (AD) involves severe impairment of cognitive and executive functions and
represents 60-70% of all cases of dementia. Neuropathologically, it is characterized by the
deposition of beta-amyloid peptide in extracellular neuritic plaques and the formation of
intraneuronal neurofibrillary tangles, which elimination has been the unsuccessful goal of different
therapies. However, little is known about the progressing neuroinflammatory process,
characterized by an increase in the number and morphological changes of microglial cells at
different stages of the disease. The aim of this study is to characterize the morphological
differences of microglial cells in the hippocampus of an aged murine model of Alzheimer's disease
to elucidate whether, associated with age and tau and beta amyloid deposits, there is an active
proinflammatory phenotype different from the physiological pattern and how it is related to the
formation of tau neurofibrillary tangles.
Twelve female mice (healthy controls and 3xTgAD, n = 6 per group) between 19 and 22 months
were used and 10 cells from each animal were randomly analysed using the AnalyzeSkeleton and
FracLac extensions of the Image-J program. No significant differences were found between both
groups. Secondly, we studied microglial relationship with tau accumulation in controls and 3xTgAD
animals at different stages (9, 12 and 15 months, n=3). Our results show an increase in both the
number of microglia cells and intracellular tau tangles associated with age, as well as a strong
relationship between both variables.
In sum, we must consider the age variable to understand the rol of microglial cells in AD
neurodegeneration process. Understanding their morphological heterogeneity may be one of the
keys of this neurodegenerative disorder. [-]
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Descripció
Treball Final de Màster Universitari en Investigació en Cervell i Conducta. Codi: SBM024. Curs: 2020/2021
Tipus de document
info:eu-repo/semantics/masterThesisDrets d'accés
info:eu-repo/semantics/openAccess