Protein adsorption/desorption dynamics on Ca‑enriched titanium surfaces: biological implications
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Other documents of the author: Romero-Gavilán, Francisco J; Cerqueira, Andreia; Anitua, Eduardo; Tejero, Ricardo; García-Arnáez, Iñaki; Martinez Ramos, Cristina; Ozturan, Seda; Izquierdo Escrig, Raul; Azkargorta, Mikel; Elortza, Felix; GURRUCHAGA, MARILO; Goñi, Isabel; Suay, Julio
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comunitat-uji-handle2:10234/7034
comunitat-uji-handle3:10234/8619
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INVESTIGACIONMetadata
Title
Protein adsorption/desorption dynamics on Ca‑enriched titanium surfaces: biological implicationsAuthor (s)
Date
2021-08-27Publisher
SpringerISSN
0949-8257; 1432-1327Bibliographic citation
Romero-Gavilán, F., Cerqueira, A., Anitua, E. et al. Protein adsorption/desorption dynamics on Ca-enriched titanium surfaces: biological implications. J Biol Inorg Chem 26, 715–726 (2021). https://doi.org/10.1007/s00775-021-01886-4Type
info:eu-repo/semantics/articlePublisher version
https://www.springer.com/journal/775Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Calcium ions are used in the development of biomaterials for the promotion of coagulation, bone regeneration, and implant osseointegration. Upon implantation, the time-dependent release of calcium ions from titanium ... [+]
Calcium ions are used in the development of biomaterials for the promotion of coagulation, bone regeneration, and implant osseointegration. Upon implantation, the time-dependent release of calcium ions from titanium implant surfaces modifies the physicochemical characteristics at the implant–tissue interface and thus, the biological responses. The aim of this study is to examine how the dynamics of protein adsorption on these surfaces change over time. Titanium discs with and without Ca were incubated with human serum for 2 min, 180 min, and 960 min. The layer of proteins attached to the surface was characterised using nLC-MS/MS. The adsorption kinetics was different between materials, revealing an increased adsorption of proteins associated with coagulation and immune responses prior to Ca release. Implant–blood contact experiments confirmed the strong coagulatory effect for Ca surfaces. We employed primary human alveolar osteoblasts and THP-1 monocytes to study the osteogenic and inflammatory responses. In agreement with the proteomic results, Ca-enriched surfaces showed a significant initial inflammation that disappeared once the calcium was released. The distinct protein adsorption/desorption dynamics found in this work demonstrated to be useful to explain the differential biological responses between the titanium and Ca-ion modified implant surfaces. [-]
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JBIC Journal of Biological Inorganic Chemistry volume 26, pages715–726 (2021)Related data
https://static-content.springer.com/esm/art%3A10.1007%2Fs00775-021-01886-4/MediaObjects/775_2021_1886_MOESM1_ESM.pdfFunder Name
Ministerio de Asuntos Económicos y Transformación Digital | Generalitat Valenciana | Universitat Jaume I | Universidad del País Vasco | Gobierno Vasco
Project code
MAT2017-86043-R | GRISOLIAP/2018/091 | RTC-2017-6147-1 | APOSTD/2020/036 | PROMETEO/2020/069 | UJI-B2017-37 | GIU18/189 | PRE_2017_2_0044
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© Author(s) 2021
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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