Analytical Strategy for Identification and Quantification of 13 Steroids in Sole (Solea senegalensis) Tissues, Eggs, and Larvae for Application in Aquaculture Studies of Reproduction
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Metadades
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INVESTIGACIONMetadades
Títol
Analytical Strategy for Identification and Quantification of 13 Steroids in Sole (Solea senegalensis) Tissues, Eggs, and Larvae for Application in Aquaculture Studies of ReproductionData de publicació
2021Editor
American Chemical SocietyISSN
2692-1952Cita bibliogràfica
Serrano, R.; Beltrán, E.; Piquer, V.; Hernández, F.; Mañanós, E. Analytical Strategy for Identification and Quantification of 13 Steroids in Sole (Solea senegalensis) Tissues, Eggs, and Larvae for Application in Aquaculture Studies of Reproduction. ACS Agric. Sci. Technol. 2021Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
https://pubs.acs.org/doi/full/10.1021/acsagscitech.0c00075Versió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
Knowing the levels of steroids in fish is crucial for understanding the reproductive processes during fish development and for reproductive success in aquaculture facilities. Although some of these compounds are present ... [+]
Knowing the levels of steroids in fish is crucial for understanding the reproductive processes during fish development and for reproductive success in aquaculture facilities. Although some of these compounds are present at very low concentrations, they play a relevant role in reproduction processes. Therefore, a very sensitive and reliable analytical methodology is required for their accurate determination. In this work, ultra-high-performance liquid chromatography coupled to tandem mass spectrometry with a triple quadrupole has been optimized for the identification and quantification of 13 steroids in plasma, muscle, and ovaries of sole (Solea senegalensis) and, for the first time, in fish eggs and larvae. This is noteworthy because studying fish development in its early stages could be the key to solving the reproductive problems observed in farmed specimens. Different strategies have been applied to solve analytical challenges such as matrix effects or the small amount of sample available. Compounds selected include progestagens (progesterone, 17α,20β-dihydroxypregnenone, and 17α,20β,21-trihydroxypregnenone), androgens (androstenedione, 11β-hydroxyandrostenedione, 11-ketoandrostenedione, testosterone, 5α-dihydrotestosterone, and 11-ketotestosterone), estrogens (estrone and estradiol), and corticosteroids (cortisol and 11-deoxycortisol). The methodology was validated in terms of accuracy (between 73% and 131%) and precision (relative standard deviations of <24%), at concentrations as low as 0.1 ng/mL for plasma, 0.1 ng/g for muscle and eggs, and 0.5 ng/g for ovaries and larvae. The application of the developed methodology to the analysis of samples from captive fish has allowed the identification and quantification of steroids at sub-nanogram per millilier and sub-nanogram per gram levels. [-]
Publicat a
ACS Agricultural Science & Technology, 2021Entitat finançadora
Ministerio de Economía y Competitividad | Research Unit of Marine Ecotoxicology | Generalitat Valenciana
Codi del projecte o subvenció
AGL2013-49027-C3-3-R | IATS-CSIC/IUPA-UJI | Prometeo/2019/040
Títol del projecte o subvenció
Ritmos embrionarios y larvarios: papel de los ciclos ambientales en el desarrollo. Determinación/diferenciación sexual y reproducción del lenguado
Drets d'accés
info:eu-repo/semantics/openAccess
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