1-Methyltryptophan Treatment Increases Defense-Related Proteins in the Apoplast of Tomato Plants
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Altres documents de l'autoria: Llorens, Eugenio; Scalschi, Loredana; González-Hernández, Ana I.; Camañes, Gemma; García Agustín, Pilar; Vicedo, Begonya
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Mostra el registre complet de l'elementcomunitat-uji-handle:10234/9
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comunitat-uji-handle3:10234/6999
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Títol
1-Methyltryptophan Treatment Increases Defense-Related Proteins in the Apoplast of Tomato PlantsAutoria
Data de publicació
2020-09-29Editor
American Chemical SocietyISSN
1535-3893Cita bibliogràfica
LLORENS, Eugenio, et al. 1-Methyltryptophan Treatment Increases Defense-Related Proteins in the Apoplast of Tomato Plants. Journal of Proteome Research, 2020.Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
https://pubs.acs.org/doi/10.1021/acs.jproteome.0c00498Versió
info:eu-repo/semantics/acceptedVersionParaules clau / Matèries
Resum
The activation of induced resistance in plants may enhance the production of defensive proteins to avoid the invasion of pathogens. In this way, the composition of the apoplastic fluid could represent an important ... [+]
The activation of induced resistance in plants may enhance the production of defensive proteins to avoid the invasion of pathogens. In this way, the composition of the apoplastic fluid could represent an important layer of defense that plants can modify to avoid the attack. In this study, we performed a proteomic study of the apoplastic fluid from plants treated with the resistance inducer 1-methyltryptophan (1-MT) as well as infected with Pseudomonas syringae pv. tomato (Pst). Our results showed that both the inoculation with Pst and the application of the inducer provoke changes in the proteomic composition in the apoplast enhancing the accumulation of proteins involved in plant defense. Finally, one of the identified proteins that are overaccumulated upon the treatment have been expressed in Escherichia coli and purified in order to test their antimicrobial effect. The result showed that the tested protein is able to reduce the growth of Pst in vitro. Taken together, in this work, we described the proteomic changes in the apoplast induced by the treatment and by the inoculation, as well as demonstrated that the proteins identified have a role in the plant protection. [-]
Proyecto de investigación
(AGL2013-49023-C03-02-R and AGL2017-85987-C3-1-R), (P1·1B2013-75, UJI-B2017-30, and UJI-A2016-09)Drets d'accés
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