The Histone Marks Signature in Exonic and Intronic Regions Is Relevant in Early Response of Tomato Genes to Botrytis cinerea and in miRNA Regulation
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Otros documentos de la autoría: Crespo, Óscar; Sánchez-Giménez, Lorena; López-Galiano, María José; Fernández Crespo, Emma; Scalschi, Loredana; Garcia-Robles, Inmaculada; Rausell, Carolina; Real, María Dolores; González-Bosch, Carmen
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Título
The Histone Marks Signature in Exonic and Intronic Regions Is Relevant in Early Response of Tomato Genes to Botrytis cinerea and in miRNA RegulationAutoría
Fecha de publicación
2020Editor
MDPIISSN
2223-7747Cita bibliográfica
Crespo-Salvador, Ó.; Sánchez-Giménez, L.; López-Galiano, M.J.; Fernández-Crespo, E.; Scalschi, L.; García-Robles, I.; Rausell, C.; Real, M.D.; González-Bosch, C. The Histone Marks Signature in Exonic and Intronic Regions Is Relevant in Early Response of Tomato Genes to Botrytis cinerea and in miRNA Regulation. Plants 2020, 9, 300.Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.mdpi.com/2223-7747/9/3/300Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Research into the relationship between epigenetic regulation and resistance to biotic stresses
provides alternatives for plant protection and crop improvement. To unravel the mechanisms
underlying tomato responses ... [+]
Research into the relationship between epigenetic regulation and resistance to biotic stresses
provides alternatives for plant protection and crop improvement. To unravel the mechanisms
underlying tomato responses to Botrytis cinerea, we performed a chromatin immunoprecipitation
(ChIP) analysis showing the increase in H3K9ac mark along the early induced genes SlyDES, SlyDOX1,
and SlyLoxD encoding oxylipin-pathway enzymes, and SlyWRKY75 coding for a transcriptional
regulator of hormonal signaling. This histone mark showed a more distinct distribution than the
previously studied H3K4me3. The RNAPol-ChIP analysis reflected the actual gene transcription
associated with increased histone modifications. A different pattern of marks in the oxylipin-related
genes against P. syringae supported a pathogen-specific profile, while no significant differences
occurred in SlyWRKY75. The epigenetic regulation of SlyWRKY75 by the intron-binding miR1127-3p
was supported by the presence of SlyWRKY75 pre-mRNA in control plants. Interestingly, mRNA was
found to be accumulated in response to B. cinerea and P. syringae, while reduction in miRNA only
occurred against B. cinerea. The intronic region presented a similar pattern of marks than the rest
of the gene in both pathosystems, except for H3K4me3 in the miRNA binding site upon B. cinerea.
We located the gene encoding Sly-miR1127-3p, which presented reduced H3K4me3 on its promoter
against B. cinerea. [-]
Publicado en
Plants 2020, 9, 300.Proyecto de investigación
AGL2013-49023-C3, AGL2017-85987-C3, AGL2013-49023-C3-1-R, AGL2017-85987-C3-2-RDerechos de acceso
info:eu-repo/semantics/openAccess
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