Quantification of rifampicin and rifabutin in plasma of tuberculosis patients by micellar liquid chromatography
Impact
![Google Scholar](/xmlui/themes/Mirage2/images/uji/logo_google.png)
![Microsoft Academico](/xmlui/themes/Mirage2/images/uji/logo_microsoft.png)
Metadata
Show full item recordcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
comunitat-uji-handle4:
INVESTIGACIONThis resource is restricted
https://doi.org/10.1016/j.microc.2020.104865 |
Metadata
Title
Quantification of rifampicin and rifabutin in plasma of tuberculosis patients by micellar liquid chromatographyAuthor (s)
Date
2020Publisher
ElsevierISSN
0026-265XBibliographic citation
BRAVO, Maria Ángeles Goberna, et al. Quantification of Rifampicin and Rifabutin in Plasma of Tuberculosis Patients by Micellar Liquid Chromatography. Microchemical Journal, 2020, p. 104865.Type
info:eu-repo/semantics/articlePublisher version
https://www.sciencedirect.com/science/article/pii/S0026265X20307165Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
A Micellar Liquid Chromatographic method is described to determine Rifampicin and Rifabutin in plasma from
Tuberculosis patients. Samples were diluted in mobile phase and then directly injected, avoiding long and ... [+]
A Micellar Liquid Chromatographic method is described to determine Rifampicin and Rifabutin in plasma from
Tuberculosis patients. Samples were diluted in mobile phase and then directly injected, avoiding long and tedious extraction steps. The analytes were resolved from the matrix without interferences from endogenous
compounds using a mobile phase of sodium dodecyl sulfate 0.15 mol L-1–6%(v/v) 1-pentanol and phosphate
buffer at pH 3, running at 1 mL min−1 through a C18 column at 25 °C. Detection was carried out by UV
absorbance at 270 nm. Under these conditions, the final chromatographic analysis time was 22 min. The analytical methodology was validated following the FDA 2018 Bioanalytical Method Validation Guidance for
Industry. The response of the drugs in plasma was linear in the 0.05–5 μg/mL range, with r
2 > 0.9993. Trueness
and precision were <14% for both substances. Carry over and matrix effects were negligible. Dilution integrity,
robustness and stability were also investigated. Method was reliable, economic, eco-friendly, safe, easy-toconduct, and with a high sample throughput, thus useful for routine analysis. Finally, the analytical method was
used to determine both antituberculosis drugs in incurred plasma samples of Tuberculosis patients. [-]
Is part of
Microchemical Journal, 157, 2020.Investigation project
UJIB2-018-20, AICO/2017/063Rights
0026-265X/ © 2020 Elsevier B.V. All rights reserved.
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/restrictedAccess
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/restrictedAccess
This item appears in the folowing collection(s)
- QFA_Articles [825]