Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals
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Other documents of the author: Pawar, Rajendra-Prasad; Mishra, Pooja; Durgbanshi, Abhilasha; Bose, Devasish; Albiol Chiva, Jaume; Vives-Peris, Vicente; García Ferrer, Daniel; Esteve-Romero, Josep
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Show full item recordcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
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Title
Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine AnimalsAuthor (s)
Date
2020-02-14Publisher
MDPIISSN
2079-6382Bibliographic citation
Prasad Pawar, R.; Mishra, P.; Durgbanshi, A.; Bose, D.; Albiol-Chiva, J.; Peris-Vicente, J.; García-Ferrer, D.; Esteve-Romero, J. Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals. Antibiotics 2020, 9, 86.Type
info:eu-repo/semantics/articlePublisher version
https://www.mdpi.com/2079-6382/9/2/86/htmVersion
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Abstract
Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was ... [+]
Mebendazole is an anthelmintic drug used in cattle production. However, residues may occur in produced food and in excretions, jeopardizing population health. A method based on micellar liquid chromatography (MLC) was developed to determine mebendazole in dairy products (milk, cheese, butter, and curd) and nitrogenous waste (urine and dung) from bovine animals. Sample treatment was expedited to simple dilution or solid-to-liquid extraction, followed by filtration and direct injection of the obtained solution. The analyte was resolved from matrix compounds in less than 8 min, using a C18 column and a mobile phase made up of 0.15 M sodium dodecyl sulfate (SDS)–6% 1-pentanol phosphate buffered at pH 7, and running at 1 mL/min under isocratic mode. Detection was performed by absorbance at 292 nm. The procedure was validated according to the guidelines of the EU Commission Decision 2002/657/EC in terms of: specificity, method calibration range (from the limit of quantification to 25–50 ppm), sensitivity (limit of detection 0.1–0.2 ppm; limit of quantification, 0.3–0.6 ppm), trueness (92.5–102.3%), precision (<7.5%, expressed at RSD), robustness, and stability. The method is reliable, sensitive, easy-to-handle, eco-friendly, safe, inexpensive, and provides a high sample-throughput. Therefore, it is useful for routine analysis as a screening or quantification method in a laboratory for drug-residue control. [-]
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Antibiotics 2020, 9(2)Investigation project
Regional Valencian Government “Generalitat Valenciana: AICO/2017/063; University Jaume I : UJI-B2018-20Rights
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