Conduits based on the combination of hyaluronic acid and silk fibroin: Characterization, in vitro studies and in vivo biocompatibility
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Title
Conduits based on the combination of hyaluronic acid and silk fibroin: Characterization, in vitro studies and in vivo biocompatibilityAuthor (s)
Date
2020-01-16Publisher
ElsevierBibliographic citation
ROCA, F. Gisbert, et al. Conduits based on the combination of hyaluronic acid and silk fibroin: Characterization, in vitro studies and in vivo biocompatibility. International Journal of Biological Macromolecules, 2020, 148: 378-390.Type
info:eu-repo/semantics/articlePublisher version
https://www.sciencedirect.com/science/article/pii/S0141813019370825Version
info:eu-repo/semantics/submittedVersionSubject
Abstract
We address the production of structures intended as conduits made from natural biopolymers, capable of promoting the regeneration of axonal tracts. We combine hyaluronic acid (HA) and silk fibroin (SF) with the aim ... [+]
We address the production of structures intended as conduits made from natural biopolymers, capable of promoting the regeneration of axonal tracts. We combine hyaluronic acid (HA) and silk fibroin (SF) with the aim of improving mechanical and biological properties of HA. The results show that SF can be efficiently incorporated into the production process, obtaining conduits with tubular structure with a matrix of HA-SF blend. HA-SF has better mechanical properties than sole HA, which is a very soft hydrogel, facilitating manipulation. Culture of rat Schwann cells shows that cell adhesion and proliferation are higher than in pure HA, maybe due to the binding motifs contributed by the SF protein. This increased proliferation accelerates the formation of a tight cell layer, which covers the inner channel surface of the HA-SF tubes. Biocompatibility of the scaffolds was studied in immunocompetent mice. Both HA and HA-SF scaffolds were accepted by the host with no residual immune response at 8 weeks. New collagen extracellular matrix and new blood vessels were visible and they were present earlier when SF was present. The results show that incorporation of SF enhances the mechanical properties of the materials and results in promising biocompatible conduits for tubulization strategies. [-]
Investigation project
Spanish Ministry of Economy and Competitiveness (grants RTI2018-095872-B-C22/ERDF, DPI2015-72863-EXP, MAT2016-79832-R and MAT2016-76847-R) ; Community of Madrid (grant Neurocentro-B2017/BMD-3760) ; Spanish Ministry of Education, Culture and Sports (FPU16/01833).Rights
© 2020 Published by Elsevier B.V.
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