Disruption of gut integrity and permeability contributes to enteritis in a fsh‑parasite model: a story told from serum metabolomics
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Other documents of the author: Sitjà-Bobadilla, Ariadna; Gil Solsona, Ruben; Estensoro, Itziar; Piazzon, M. Carla; Martos-Sitcha, Juan Antonio; Picard‑Sánchez, Amparo; Fuentes, Juan; Vicente Sancho, Juan; Calduch-Giner, Josep; Hernandez, Felix; Pérez Sánchez, Jaume
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comunitat-uji-handle2:10234/33596
comunitat-uji-handle3:10234/33597
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Title
Disruption of gut integrity and permeability contributes to enteritis in a fsh‑parasite model: a story told from serum metabolomicsAuthor (s)
Date
2019Publisher
BMCISSN
1756-3305Bibliographic citation
SITJÀ-BOBADILLA, Ariadna, et al. Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics. Parasites & vectors, 2019, vol. 12, no 1, p. 486.Type
info:eu-repo/semantics/articlePublisher version
https://parasitesandvectors.biomedcentral.com/articles?query=Disruption+of+gut+i ...Version
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Abstract
Background: In the animal production sector, enteritis is responsible for serious economic losses, and intestinal
parasitism is a major stress factor leading to malnutrition and lowered performance and animal production ... [+]
Background: In the animal production sector, enteritis is responsible for serious economic losses, and intestinal
parasitism is a major stress factor leading to malnutrition and lowered performance and animal production efciency.
The efect of enteric parasites on the gut function of teleost fsh, which represent the most ancient bony vertebrates,
is far from being understood. The intestinal myxozoan parasite Enteromyxum leei dwells between gut epithelial cells
and causes severe enteritis in gilthead sea bream (Sparus aurata), anorexia, cachexia, growth impairment, reduced
marketability and increased mortality.
Methods: This study aimed to outline the gut failure in this fsh-parasite model using a multifaceted approach and
to fnd and validate non-lethal serum markers of gut barrier dysfunction. Intestinal integrity was studied in parasitized
and non-parasitized fsh by immunohistochemistry with specifc markers for cellular adhesion (E-cadherin) and tight
junctions (Tjp1 and Cldn3) and by functional studies of permeability (oral administration of FITC-dextran) and electro‑
physiology (Ussing chambers). Serum samples from parasitized and non-parasitized fsh were analyzed using non-tar‑
geted metabolomics and some signifcantly altered metabolites were selected to be validated using commercial kits.
Results: The immunodetection of Tjp1 and Cldn3 was signifcantly lower in the intestine of parasitized fsh, while
no strong diferences were found in E-cadherin. Parasitized fsh showed a signifcant increase in paracellular uptake
measured by FITC-dextran detection in serum. Electrophysiology showed a decrease in transepithelial resistance in
infected animals, which showed a diarrheic profle. Serum metabolomics revealed 3702 ions, from which the dif‑
ferential expression of 20 identifed compounds signifcantly separated control from infected groups in multivariate
analyses. Of these compounds, serum inosine (decreased) and creatine (increased) were identifed as relevant and
validated with commercial kits.
Conclusions: The results demonstrate the disruption of tight junctions and the loss of gut barrier function, a metab‑
olomic profle of absorption dysfunction and anorexia, which further outline the pathophysiological efects of E. leei. [-]
Is part of
Parasites Vectors (2019) 12:486Investigation project
H2020-634429 ; 652831 TNA AE10004-INTEBREAM ; AGL2013-48560-R ; 201740E013 ; APOSTD/2016/037 ; UID/Multi 04326/2019Rights
info:eu-repo/semantics/openAccess
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