Encoding of arbitrary micrometric complex illumination patterns with reduced speckle
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Other documents of the author: Carbonell Leal, Miguel; Mínguez-Vega, Gladys; Lancis, Jesús; Mendoza-Yero, Omel
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Title
Encoding of arbitrary micrometric complex illumination patterns with reduced speckleDate
2019Publisher
Optical Society of AmericaISSN
1094-4087Bibliographic citation
Miguel Carbonell-Leal, Gladys Mínguez-Vega, Jesús Lancis, and Omel Mendoza-Yero, "Encoding of arbitrary micrometric complex illumination patterns with reduced speckle," Opt. Express 27, 19788-19801 (2019)Type
info:eu-repo/semantics/articleVersion
info:eu-repo/semantics/publishedVersionAbstract
In nonlinear microscopy, phase-only spatial light modulators (SLMs) allow
achieving simultaneous two-photon excitation and fluorescence emission from specific regionof-interests (ROIs). However, as iterative Fourier ... [+]
In nonlinear microscopy, phase-only spatial light modulators (SLMs) allow
achieving simultaneous two-photon excitation and fluorescence emission from specific regionof-interests (ROIs). However, as iterative Fourier transform algorithms (IFTAs) can only
approximate the illumination of selected ROIs, both image formation and/or signal acquisition
can be largely affected by the spatial irregularities of the illumination patterns and the speckle
noise. To overcome these limitations, we propose an alternative complex illumination method
(CIM) able to generate simultaneous excitation of large-area ROIs with full control over the
amplitude and phase of light and reduced speckle. As a proof-of-concept we experimentally
demonstrate single-photon and second harmonic generation (SHG) with structured
illumination over large-area ROIs. [-]
Is part of
Optics Express, Vol. 27, No. 14 (2019)Investigation project
AICO/2016/036 PROMETEO 2016-079 ; UJIB2016-19 ; FIS2016-75618-RRights
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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