Identification of Stress Associated microRNAs in Solanum lycopersicum by High-Throughput Sequencing
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comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
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Title
Identification of Stress Associated microRNAs in Solanum lycopersicum by High-Throughput SequencingAuthor (s)
Date
2019-06-21Publisher
MDPIBibliographic citation
LÓPEZ-GALIANO, María José; SENTANDREU, Vicente; MARTÍNEZ RAMÍREZ, Amparo C.; RAUSELL, Carolina; REAL, María Dolores; CAMAÑES, Gemma; RUIZ-RIVERO, Omar; CRESPO SALVADOR, Óscar; GARCÍA-ROBLES, Inmaculada (2019). Identification of Stress Associated microRNAs in Solanum lycopersicum by High-Throughput Sequencing. Genes, v. 10, issue 6Type
info:eu-repo/semantics/articlePublisher version
https://www.mdpi.com/2073-4425/10/6/475Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Tomato (Solanum lycopersicum) is one of the most important crops around the world
and also a model plant to study response to stress. High-throughput sequencing was used to
analyse the microRNA (miRNA) profile of ... [+]
Tomato (Solanum lycopersicum) is one of the most important crops around the world
and also a model plant to study response to stress. High-throughput sequencing was used to
analyse the microRNA (miRNA) profile of tomato plants undergoing five biotic and abiotic stress
conditions (drought, heat, P. syringae infection, B. cinerea infection, and herbivore insect attack with
Leptinotarsa decemlineata larvae) and one chemical treatment with a plant defence inducer, hexanoic
acid. We identified 104 conserved miRNAs belonging to 37 families and we predicted 61 novel tomato
miRNAs. Among those 165 miRNAs, 41 were stress-responsive. Reverse transcription quantitative
PCR (RT-qPCR) was used to validate high-throughput expression analysis data, confirming the
expression profiles of 10 out of 11 randomly selected miRNAs. Most of the differentially expressed
miRNAs were stress-specific, except for sly-miR167c-3p upregulated in B. cinerea and P. syringae
infection, sly-newmiR26-3p upregulated in drought and Hx treatment samples, and sly-newmiR33-3p,
sly-newmiR6-3p and sly-newmiR8-3p differentially expressed both in biotic and abiotic stresses.
From mature miRNAs sequences of the 41 stress-responsive miRNAs 279 targets were predicted.
An inverse correlation between the expression profiles of 4 selected miRNAs (sly-miR171a, sly-miR172c,
sly-newmiR22-3p and sly-miR167c-3p) and their target genes (Kinesin, PPR, GRAS40, ABC transporter,
GDP and RLP1) was confirmed by RT-qPCR. Altogether, our analysis of miRNAs in different biotic
and abiotic stress conditions highlight the interest to understand the functional role of miRNAs in
tomato stress response as well as their putative targets which could help to elucidate plants molecular
and physiological adaptation to stress. [-]
Is part of
Genes (2019), v. 10, issue 6Investigation project
1) Supported by “MINECO” and “FEDER” (projects AGL2013-49023-C3-3-R, AGL2017-85987-C3-3-R); 2) Grant AGL2013-49023-C03-0Rights
info:eu-repo/semantics/openAccess
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