Complement proteins regulating macrophage polarisation on biomaterials
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Título
Complement proteins regulating macrophage polarisation on biomaterialsAutoría
Fecha de publicación
2019-09-01Editor
ElsevierISSN
0927-7765Cita bibliográfica
ARAÚJO-GOMES, N., et al. Complement proteins regulating macrophage polarisation on biomaterials. Colloids and Surfaces B: Biointerfaces, 2019, vol. 181, p. 125-133Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.sciencedirect.com/science/article/pii/S0927776519303479?via%3Dihub#!Versión
info:eu-repo/semantics/submittedVersionPalabras clave / Materias
Resumen
One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, ... [+]
One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organically-modified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that greater GPTMS surface concentrations induced greater secretion of TNF-α and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP) and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentration-dependent. Our results show that a greater adsorption of complement proteins can condition macrophage polarization. [-]
Publicado en
Colloids and Surfaces B: Biointerfaces, 2019, vol. 181Proyecto de investigación
MINECO: MAT2017-86043-R]; Universitat Jaume I: Predoc/2014/25, UJI-B2017-37; Basque Government : IT611-13, Predoc/2016/1/0141]; University of the Basque Country [UFI11/56]; CIC bioGUNE is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), ProteoRed-ISCIII: PRB3 IPT17/0019; CIBERehd Network; Severo Ochoa Grant: SEV-2016-0644]Derechos de acceso
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