Re-certification of hydroxyvitamin D standards by isotope pattern deconvolution
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Otros documentos de la autoría: Pitarch-Motellón, Jorge; Roig-Navarro, Antoni F.; Le Goff, C.; Cavalier, E.; Fabregat-Cabello, Neus
Metadatos
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INVESTIGACIONMetadatos
Título
Re-certification of hydroxyvitamin D standards by isotope pattern deconvolutionAutoría
Fecha de publicación
2019-04-22Editor
ElsevierCita bibliográfica
PITARCH-MOTELLÓN, J., et al. Re-certification of hydroxyvitamin D standards by isotope pattern deconvolution. Journal of Chromatography B, 2019, 1120: 89-94Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.sciencedirect.com/science/article/pii/S1570023219301461Versión
info:eu-repo/semantics/acceptedVersionPalabras clave / Materias
Resumen
Background
Vitamin D testing in analytical clinical laboratories has been experiencing a rapid increase of demand over the last years, as it plays a key role in several disorders. Due to the narrow ranges of medical ... [+]
Background
Vitamin D testing in analytical clinical laboratories has been experiencing a rapid increase of demand over the last years, as it plays a key role in several disorders. Due to the narrow ranges of medical significance regarding its concentration levels in human serum, accurate and precise determinations of vitamin D metabolites are required.
Methods
We present an isotope dilution mass spectrometry quantification method for the re-certification of routine commercial standards used in method validation steps, isotope pattern deconvolution (IPD) based on LC-MS/MS.
Results
IPD allowed to compensate for the observed biases of +4.7% for 25(OH)D3, −29% for 25(OH)D2 and −30% for 24,25(OH)2D3 standard concentrations, respectively in an easy, cheap and straightforward way.
Conclusions
Is has been observed that, in some cases, discrepancies may exist between stated purity or amount of routinely used commercial standards and actual values, which would lead to unwanted bias in the developed methodologies. The present correction has helped meeting the regulations established by international standardization programs, including Vitamin D Standardization Program (VDSP). [-]
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© 2019 Elsevier B.V. All rights reserved.
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info:eu-repo/semantics/openAccess
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