Bioactive potential of silica coatings and its effect on the adhesion of proteins to titanium implants
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Other documents of the author: Romero-Gavilán, Francisco J; Gomes, Nuno; Sánchez-Pérez, Ana María; García-Arnáez, Iñaki; Elortza, Felix; Azkargorta, Mikel; Martín de Llano, J.J.; Carda, C.; Gurruchaga, Marilo; Suay, Julio; Goñi, Isabel
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comunitat-uji-handle2:10234/7034
comunitat-uji-handle3:10234/8619
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INVESTIGACIONMetadata
Title
Bioactive potential of silica coatings and its effect on the adhesion of proteins to titanium implantsAuthor (s)
Date
2018-02-01Publisher
ElsevierISSN
0927-7765; 1873-4367Bibliographic citation
ROMERO-GAVILAN, F., et al. Bioactive potential of silica coatings and its effect on the adhesion of proteins to titanium implants. Colloids and Surfaces B: Biointerfaces, 2018, vol. 162, p. 316-325.Type
info:eu-repo/semantics/articlePublisher version
https://www.sciencedirect.com/science/article/pii/S0927776517308378Version
info:eu-repo/semantics/submittedVersionSubject
Abstract
There is an ever-increasing need to develop dental implants with ideal characteristics to achieve specific and desired biological response in the scope of improve the healing process post-implantation. Following that ... [+]
There is an ever-increasing need to develop dental implants with ideal characteristics to achieve specific and desired biological response in the scope of improve the healing process post-implantation. Following that premise, enhancing and optimizing titanium implants through superficial treatments, like silica sol-gel hybrid coatings, are regarded as a route of future research in this area. These coatings change the physicochemical properties of the implant, ultimately affecting its biological characteristics. Sandblasted acid-etched titanium (SAE-Ti) and a silica hybrid sol-gel coating (35M35G30T) applied onto the Ti substrate were examined. The results of in vitro and in vivo tests and the analysis of the protein layer adsorbed to each surface were compared and discussed.
In vitro analysis with MC3T3-E1 osteoblastic cells, showed that the sol-gel coating raised the osteogenic activity potential of the implants (the expression of osteogenic markers, the alkaline phosphatase (ALP) and IL-6 mRNAs, increased). In the in vivo experiments using as model rabbit tibiae, both types of surfaces promoted osseointegration. However, the coated implants demonstrated a clear increase in the inflammatory activity in comparison with SAE-Ti. Mass spectrometry (LC–MS/MS) analysis showed differences in the composition of protein layers formed on the two tested surfaces. Large quantities of apolipoproteins were found attached predominantly to SAE-Ti. The 35M35G30T coating adsorbed a significant quantity of complement proteins, which might be related to the material intrinsic bioactivity, following an associated, natural and controlled immune response.
The correlation between the proteomic data and the in vitro and in vivo outcomes is discussed on this experimental work. [-]
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Colloids and Surfaces B: Biointerfaces, 2018, vol. 162Investigation project
MINECO: MAT 2014-51918-C2-2-R; Universidad Jaume I: Predoc/2014/25; Generalitat Valenciana: Grisolia/2014/016; Basque Government: Pre-doct/2016/1/0141, P11B2014-19Rights
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