Thermally Regulated Reversible Formation of Vesicle-Like Assemblies by Hexaproline Amphiphiles
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INVESTIGACIONMetadata
Title
Thermally Regulated Reversible Formation of Vesicle-Like Assemblies by Hexaproline AmphiphilesAuthor (s)
Date
2017-07Publisher
American Chemical SocietyBibliographic citation
FELIP-LEÓN, Carles, et al. Thermally Regulated Reversible Formation of Vesicle-Like Assemblies by Hexaproline Amphiphiles. The Journal of Physical Chemistry B, 2017, vol. 121, no 31, p. 7443-7446.Type
info:eu-repo/semantics/articlePublisher version
http://pubs.acs.org/doi/abs/10.1021/acs.jpcb.7b06167Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Peptides composed of hexaproline and glutamic acid (P6E) or lysine (P6K) as C-terminal units show thermally promoted aggregation, affording vesicle-like assemblies upon heating to 80 °C. The aggregation is analyzed ... [+]
Peptides composed of hexaproline and glutamic acid (P6E) or lysine (P6K) as C-terminal units show thermally promoted aggregation, affording vesicle-like assemblies upon heating to 80 °C. The aggregation is analyzed by dynamic light scattering (DLS), with number-averaged diameters of ca. 600 and 300 nm, respectively, for P6E and P6K. NMR studies reveal that upon heating the amount of NMR-visible species is reduced to ca. 50% and that an important conformational change is experienced by the molecules in solution. Circular dichroism (CD) shows that at 20 °C the peptides present a polyproline II (PP-II) conformation which is disorganized upon heating. Scanning electron microscopy for samples which were fast frozen at 80 °C reveals vesicle-like assemblies. Using pyrene as a fluorescence probe, a critical aggregation concentration of ca. 30 μM was estimated for P6E, while that of P6K was above 0.6 mM. The aggregation process is found to be fully reversible and could serve as a basis for development of stimuli responsive carriers. [-]
Investigation project
Ministerio de Economía y Competitividad of Spain (grant CTQ2015-71004-R), Universitat Jaume I (grant P1.1B2015-76), EPSRC (UK) (Platform grant ref. EP/LP20599/1), and Government of Spain (predoctoral fellowship (BES-2013-063296)).Rights
© 2017 American Chemical Society
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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