Metabolomic approach for quality classification of olive oils by gas chromatography coupled to quadrupole time-of-flight mass spectrometry and development of an alternaitve method
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Título
Metabolomic approach for quality classification of olive oils by gas chromatography coupled to quadrupole time-of-flight mass spectrometry and development of an alternaitve methodAutoría
Tutor/Supervisor
Beltrán Arandes, JoaquimTutor/Supervisor; Universidad.Departamento
Universitat Jaume I. Departament de Química Física i AnalíticaFecha de publicación
2015-07-31Editor
Universitat Jaume IResumen
Determining the quality of olive oil has always been a conflict, as to date, the only way
that was available for classification was through tasting by a panel of experts. This
differentiation, performed by a quite ... [+]
Determining the quality of olive oil has always been a conflict, as to date, the only way
that was available for classification was through tasting by a panel of experts. This
differentiation, performed by a quite subjective method, could be complex, and sometimes
leads to large discrepancies in the organoleptic qualities of the same oil depending on the
taster to try it. Such discrepancies may result in misclassification of the oil, resulting in
economic harm to producers, to determine a quality below the real to a particular product.
The progress each day over chromatographic techniques coupled to mass spectrometry
allows to reach detect differences in concentrations of trace compounds even in the range of
parts per trillion. Through these techniques it can be quantified with high accuracy at these
levels, something impossible for a tasting panel or other physicochemical techniques.
In the same way, in highlighting differences between large groups of samples, technical
progress with high resolving power, and the development of new information technologies
has facilitated the emergence of complex statistical analyzes that allow, based a previous
model, a sample assignment within a given group with a minimum data treatment. In this field,
metabolomics is the most efficient methodology, as it allows, through statistical analysis of
individual samples, create patterns of behavior of different groups and thus classify blind
samples in each group with minimal error.
In this work, a metabolomics approach has been carried out in order to create a statistical
model to perform a classification between different quality olive oils. Hybrid quadrupole timeof-
flight mass spectrometer (QTOF MS) coupled to gas chromatography (GC) has been used
for untargeted metabolite fingerprinting. Differentiation from Extra Virgin olive oil, Virgin olive
oil and Lampante olive oil has been carried out using XCMS application and multivariate
analysis to GC-(Q)TOF MS data acquired.
Finally an alternative technique, more selective and economical also based on purge and
trap, but taking profit of the benefits of the direct thermal desorption in the injection system
of a GC, coupled to a single quadrupole (MS) has been developed. [-]
Palabras clave / Materias
Descripción
Treball Final de Grau en Química. Codi: QU0943. Curs acadèmic 2014-2015
Tipo de documento
info:eu-repo/semantics/bachelorThesisDerechos de acceso
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- Grau en Química [265]