Analysis of epinephrine, norepinephrine, and dopamine in urine samples of hospital patients by micellar liquid chromatography
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http://dx.doi.org/10.1007/s00216-015-9066-7 |
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Títol
Analysis of epinephrine, norepinephrine, and dopamine in urine samples of hospital patients by micellar liquid chromatographyAutoria
Data de publicació
2015-12-01Editor
SpringerCita bibliogràfica
GARCÍA FERRER, Daniel; GARCÍA GARCÍA, Aurelio; PERIS VICENTE, Juan; GIMENO ADELANTADO, J. V.; ESTEVE ROMERO, Josep. Analysis of epinephrine, norepinephrine, and dopamine in urine samples of hospital patients by micellar liquid chromatography. Analytical and Bioanalytical Chemistry (2015), v. 497, issue 30, pp. 9009-9018Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
http://link.springer.com/article/10.1007/s00216-015-9066-7Versió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
An analytical method based on micellar liquid chromatography was developed to determine the concentration of three catecholamines (epinephrine, norepinephrine, and dopamine) in urine. The detection of these compounds ... [+]
An analytical method based on micellar liquid chromatography was developed to determine the concentration of three catecholamines (epinephrine, norepinephrine, and dopamine) in urine. The detection of these compounds in urine can be useful to diagnose several diseases, related to stress and sympathoadrenal system dysfunction, using a non-invasive collection procedure. The sample pretreatment was a simple dilution in a micellar solution, filtration, and direct injection, thus avoiding time-consuming and tedious extraction steps. Therefore, there is no need to use an internal standard. The three catecholamines were eluted using a C18 column and a mobile phase of 0.055 M sodium dodecyl sulfate-1.5 % methanol buffered at pH 3.8 running at 1.5 mL/min under isocratic mode in less than 25 min. The detection was performed by amperometry applying a constant potential of +0.5 V. The procedure was validated following the guidelines of the European Medicines Agency in terms of the following: calibration range (0.09–5 μg/mL), linearity (r 2 > 0.9995), limit of detection (0.02 μg/mL), within- and between-run accuracy (−6.5 to +8.4 %) and precision (<10.2 %), dilution integrity, matrix effect, robustness (<8.4), and stability. The obtained values were below those required by the guide. The method was rapid, easy-to-handle, eco-friendly, and safe and provides reliable quantitative data, and is thus useful for routine analysis. The procedure was applied to the analysis of epinephrine, norepinephrine, and dopamine in urine samples from patients of a local hospital. [-]
Publicat a
Analytical and Bioanalytical Chemistry (2015), v. 497, issue 30Drets d'accés
http://rightsstatements.org/vocab/CNE/1.0/
info:eu-repo/semantics/restrictedAccess
info:eu-repo/semantics/restrictedAccess
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