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Dual-mode optical microscope based on single-pixel imaging
dc.contributor.author | Rodríguez Jiménez, Ángel David | |
dc.contributor.author | Clemente Pesudo, Pedro Javier | |
dc.contributor.author | Tajahuerce, Enrique | |
dc.contributor.author | Lancis, Jesús | |
dc.date.accessioned | 2016-04-22T07:23:07Z | |
dc.date.available | 2016-04-22T07:23:07Z | |
dc.date.issued | 2016-03 | |
dc.identifier.citation | RODRÍGUEZ, A. D., et al. Dual-mode optical microscope based on single-pixel imaging. Optics and Lasers in Engineering, 2016, 82: 87-94. | ca_CA |
dc.identifier.issn | 0143-8166 | |
dc.identifier.uri | http://hdl.handle.net/10234/158918 | |
dc.description.abstract | We demonstrate an inverted microscope that can image specimens in both reflection and transmission modes simultaneously with a single light source. The microscope utilizes a digital micromirror device (DMD) for patterned illumination altogether with two single-pixel photosensors for efficient light detection. The system, a scan-less device with no moving parts, works by sequential projection of a set of binary intensity patterns onto the sample that are codified onto a modified commercial DMD. Data to be displayed are geometrically transformed before written into a memory cell to cancel optical artifacts coming from the diamond-like shaped structure of the micromirror array. The 24-bit color depth of the display is fully exploited to increase the frame rate by a factor of 24, which makes the technique practicable for real samples. Our commercial DMD-based LED-illumination is cost effective and can be easily coupled as an add-on module for already existing inverted microscopes. The reflection and transmission information provided by our dual microscope complement each other and can be useful for imaging non-uniform samples and to prevent self-shadowing effects. | ca_CA |
dc.description.sponsorShip | This work was supported by MINECO through projects FIS2013-40666-P, the Generalitat Valenciana PROMETEO/2012/021, ISIC/2012/013, and by the Universitat Jaume I P1-1B2012-55. A.D. Rodríguez acknowledges grant PREDOC/2012/41 from Universitat Jaume I. Thanks also to Dr. Tatiana Pina and Dr. Josep Jaques from Universitat Jaume I for providing us the biological samples. | ca_CA |
dc.format.extent | 18 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Elsevier | ca_CA |
dc.relation.isPartOf | Optics and Lasers in Engineering, Volume 82, July 2016, Pages 87–94 | ca_CA |
dc.rights | Copyright © 2016 Elsevier B.V. or its licensors or contributors. | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | Microscopy | ca_CA |
dc.subject | Reflection | ca_CA |
dc.subject | Transmission | ca_CA |
dc.subject | Resolution | ca_CA |
dc.subject | Computational imaging | ca_CA |
dc.title | Dual-mode optical microscope based on single-pixel imaging | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1016/j.optlaseng.2016.02.004 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | http://www.sciencedirect.com/science/article/pii/S0143816616000348 | ca_CA |
dc.edition | Pre-print, versió de l'autor | ca_CA |
dc.type.version | info:eu-repo/semantics/submittedVersion | ca_CA |
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