Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasome
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Other documents of the author: Nieto Torres, José L.; Verdiá Báguena, Carmen; Jiménez Guardeño, José M.; Regla Nava, José A.; Castaño Rodríguez, Carlos; Fernández Delgado, Raúl; Torres, Jaume; Aguilella, Vicente; Enjuanes, Luis
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comunitat-uji-handle2:10234/2507
comunitat-uji-handle3:10234/6973
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INVESTIGACIONMetadata
Title
Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasomeAuthor (s)
Date
2015-11Publisher
ElsevierBibliographic citation
NIETO-TORRES, Jose L., et al. Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasome. Virology, 2015, vol. 485, p. 330-339.Type
info:eu-repo/semantics/articlePublisher version
http://www.sciencedirect.com/science/article/pii/S0042682215003566Version
info:eu-repo/semantics/submittedVersionSubject
SARS-CoV | Coronavirus | E protein | Ion channel | Viroporin | Calcium | Inflammasome | Pathogenesis
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) envelope (E) protein is a viroporin involved in virulence. E protein ion channel (IC) activity is specifically correlated with enhanced pulmonary damage, edema ... [+]
Severe acute respiratory syndrome coronavirus (SARS-CoV) envelope (E) protein is a viroporin involved in virulence. E protein ion channel (IC) activity is specifically correlated with enhanced pulmonary damage, edema accumulation and death. IL-1β driven proinflammation is associated with those pathological signatures, however its link to IC activity remains unknown. In this report, we demonstrate that SARS-CoV E protein forms protein–lipid channels in ERGIC/Golgi membranes that are permeable to calcium ions, a highly relevant feature never reported before. Calcium ions together with pH modulated E protein pore charge and selectivity. Interestingly, E protein IC activity boosted the activation of the NLRP3 inflammasome, leading to IL-1β overproduction. Calcium transport through the E protein IC was the main trigger of this process. These findings strikingly link SARS-CoV E protein IC induced ionic disturbances at the cell level to immunopathological consequences and disease worsening in the infected organism. [-]
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Virology Volume 485, November 2015Rights
Copyright © 2015 Elsevier B.V. All rights reserved.
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