A micellar liquid chromatography method for the quantification of abacavir, lamivudine and raltegravir in plasma
Impact
Scholar |
Other documents of the author: Vives-Peris, Vicente; Villarreal Traver, Mónica; Casas Breva, Inmaculada; Carda-Broch, Samuel; Esteve-Romero, Josep
Metadata
Show full item recordcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/7013
comunitat-uji-handle3:10234/8638
comunitat-uji-handle4:
INVESTIGACIONThis resource is restricted
http://dx.doi.org/10.1016/j.jpba.2014.06.009 |
Metadata
Title
A micellar liquid chromatography method for the quantification of abacavir, lamivudine and raltegravir in plasmaAuthor (s)
Date
2014-09Publisher
ElsevierBibliographic citation
PERIS-VICENTE, Juan, et al. A micellar liquid chromatography method for the quantification of abacavir, lamivudine and raltegravir in plasma. Journal of pharmaceutical and biomedical analysis, 2014, 98: 351-355.Type
info:eu-repo/semantics/articlePublisher version
http://www.sciencedirect.com/science/article/pii/S0731708514002970Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
An analytical methodology based on micellar liquid chromatography has been developed to quantify abacavir, lamivudine and raltegravir in plasma. These three antiretroviral drugs are prescribed as a set in highly active ... [+]
An analytical methodology based on micellar liquid chromatography has been developed to quantify abacavir, lamivudine and raltegravir in plasma. These three antiretroviral drugs are prescribed as a set in highly active antiretroviral therapy to acquired immunodeficiency syndrome patients. The experimental procedure consists in the dilution of the sample in micellar media, followed by filtration and, without cleanup step. The analytes were resolved in less than 30 min using a mobile phase of 0.05 M sodium dodecyl sulphate at pH 7, running at 1 mL min−1 under isocratic mode at room temperature through a C18 column (125 × 4.6 mm, 5 μm particle size). The UV detection wavelength was set at 260 nm. The method was successfully validated following the requirements of ICH guidelines in terms of: linear range (0.25–2.5 μg mL−1), linearity (r2 > 0.990), intra- and interday precision (<6.8%) and accuracy (92.3–104.2%) and robustness (<7.1%). To the extent of our knowledge, this is the first published method to quantify these three drugs in plasma. Several blood samples from AIDS patients taking this HAART set provided by a local hospital were analyzed with satisfactory results. [-]
Is part of
Journal of Pharmaceutical and Biomedical Analysis Volume 98, September 2014Rights
Copyright © 2014 Elsevier B.V. All rights reserved.
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/restrictedAccess
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/restrictedAccess
This item appears in the folowing collection(s)
- QFA_Articles [817]