A Fast and Precise Method To Identify Indolic Glucosinolates and Camalexin in Plants by Combining Mass Spectrometric and Biological Information
Ver/ Abrir
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/2508
comunitat-uji-handle3:10234/6999
comunitat-uji-handle4:
INVESTIGACIONMetadatos
Título
A Fast and Precise Method To Identify Indolic Glucosinolates and Camalexin in Plants by Combining Mass Spectrometric and Biological InformationFecha de publicación
2012Editor
American Chemical SocietyISSN
0021-8561; 1520-5118Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
http://pubs.acs.org/doi/pdf/10.1021/jf302482yPalabras clave / Materias
Resumen
In this manuscript, a fast and accurate identification and quantitation by mass spectrometry of indolic
glucosinolates and camalexin involved in defense in Arabidopsis thaliana are described. Two elicitation systems, ... [+]
In this manuscript, a fast and accurate identification and quantitation by mass spectrometry of indolic
glucosinolates and camalexin involved in defense in Arabidopsis thaliana are described. Two elicitation systems, inoculation with
Botrytis cinerea and treatment with AgNO3, were used in Col-0 wild-type and mutant genotypes impaired in the biosynthesis of
the selected metabolites. Identification of analytes was carried out by nontargeted LC/ESI-QTOF-MS profiling. Confirmation of
indolic glucosinolates and camalexin was achieved by their absence in the cyp79B2/B3 and pad3 mutants as well as their
respective fragmentation upon collision-induced dissociation. Camalexin accumulation was induced only after AgNO3 treatment,
whereas all indolic glucosinolates were constitutively present. Inoculation with Botrytis did not influence camalexin concentration
but caused most aliphatic and indolic glucosinolates contents to decrease. Only the pen 3.1 mutant showed increased indolic
glucosinolate levels after Botrytis or AgNO3 treatments. In addition, profiles of secondary metabolite in nontreated Col-0 and
mutant plants were analyzed by means of partial least squares coupled to discriminant analysis (PLS-DA), and differences in the
basal levels of indolic glucosinolates and tryptophan between cyp79B2/B3 plants and the rest of genotypes, including Col-0, were
found. This probably has to be taken into consideration when comparing stress responses of Col-0 and cyp79B2/B3. The use of
mutants carrying alterations in biosynthetic pathways is proposed as a useful strategy to identify secondary metabolites. [-]
Publicado en
Journal of Agricultural and Food Chemistry, 2012, vol. 60, núm. 35Derechos de acceso
This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review. To access the final edited and published work, see [http://pubs.acs.org/doi/pdf/10.1021/jf302482y].
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/openAccess
http://rightsstatements.org/vocab/InC/1.0/
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- CAMN_Articles [568]