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Fluorescence of 1,2-Diaminoanthraquinone and its Nitric Oxide Reaction Product within Macrophage Cells
dc.contributor.author | Marín, María J. | |
dc.contributor.author | Thomas, Paul | |
dc.contributor.author | Fabregat Tena, Víctor | |
dc.contributor.author | Luis, Santiago V. | |
dc.contributor.author | Russell, David A. | |
dc.contributor.author | Galindo, Francisco | |
dc.date.accessioned | 2012-11-09T14:20:33Z | |
dc.date.available | 2012-11-09T14:20:33Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | ChemBioChem (2011), 12, 16, p. 2471–2477 | ca_CA |
dc.identifier.issn | 1439-7633 | |
dc.identifier.uri | http://hdl.handle.net/10234/51559 | |
dc.description.abstract | Nitric oxide (NO) is involved in many biological processes. Aromatic ortho-diamine derivatives are commonly used in the fluorescence imaging of NO in living cells. ortho-diamino (o-diamino) compounds are believed to react with NO in an oxygenated medium leading to the formation of a triazole derivative. One such o-diamino compound, 1,2-diaminoanthraquinone (DAA), is a nontoxic probe for the detection of NO in living tissues and cells. The formation of the DAA triazole derivative (DAA-TZ) upon reaction of DAA with NO/O2 within cells has not been demonstrated previously. The aim of this study was to confirm that DAA-TZ is the species formed intracellularly when DAA reacts with NO in the presence of oxygen. The chemical synthesis and characterisation of DAA-TZ was performed together with intracellular studies of DAA and DAA-TZ. Raw 264.7 macrophages were loaded with the DAA or DAA-TZ under conditions of no-stimulation or stimulation with interferon-γ and lipopolysaccharide to produce NO. Confocal microscopy was used to image the DAA-loaded macrophage cells. Analysis of the emission spectra allowed precise discrimination of the fluorescence of each species in the macrophage cells, and confirmed the identity of DAA-TZ as the intracellular reaction product between DAA and NO in the presence of oxygen. | ca_CA |
dc.format.extent | 6 p. | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | Wiley-VCH | ca_CA |
dc.rights | Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | ca_CA |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | confocal fluorescence microscopy | ca_CA |
dc.subject | fluorescence spectroscopy | ca_CA |
dc.subject | fluorescent probes | ca_CA |
dc.subject | intracellular analysis | ca_CA |
dc.subject | nitric oxide | ca_CA |
dc.title | Fluorescence of 1,2-Diaminoanthraquinone and its Nitric Oxide Reaction Product within Macrophage Cells | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | http://dx.doi.org/10.1002/cbic.201100371 | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | ca_CA |
dc.relation.publisherVersion | http://onlinelibrary.wiley.com/doi/10.1002/cbic.201100371/abstract | ca_CA |
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