Capillary electrophoresis determination of antihistamines in serum and pharmaceuticals
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http://dx.doi.org/10.1016/j.aca.2010.03.041 |
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Títol
Capillary electrophoresis determination of antihistamines in serum and pharmaceuticalsAutoria
Data de publicació
2010Editor
ElsevierISSN
0003-2670Cita bibliogràfica
Analytica Chimica Acta, 666, 02-ene, p. 102-109Tipus de document
info:eu-repo/semantics/articleVersió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
A capillary electrophoresis (CE) procedure combined with UV detection has proved useful for the quantification of the most frequently prescribed antihistamines corresponding to the ethylendiamine, ethanolamine, ... [+]
A capillary electrophoresis (CE) procedure combined with UV detection has proved useful for the quantification of the most frequently prescribed antihistamines corresponding to the ethylendiamine, ethanolamine, propylamine, piperazine and other derivative groups in serum samples and pharmaceuticals. Discussions have focused primarily on the optimisation of the separation conditions by considering the following experimental parameters: pH, pressure injection and voltage; under the criteria of maximum resolution and minimum analysis time. The optimised parameters for the determination of antihistamines were a 24cm capillary (effective length), UV detection at 214nm, 20mM phosphate running buffer at pH 2.0, 2psis<sup>-1</sup> injection pressure and 5kV applied voltage. Under these conditions, the analysis time was below 10min. The proposed method was validated according to the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines. The limits of detection and quantification were in the ranges of 4-28 and 40-250ngL<sup>-1</sup>, respectively. Intra- and inter-day precision were tested at three different concentrations of the drugs, obtaining RSD values lower than 3% in most cases. The method was robust (RSD<5.6%), simple, specific and suitable for the practical determination of antihistamines in serum samples and pharmaceuticals with high recoveries (95.7-102.9%) without interferences. © 2010 Elsevier B.V. [-]
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