Mostrar el registro sencillo del ítem

dc.contributor.authorBeltrán Iturat, Eduardo
dc.contributor.authorIbáñez, Maria
dc.contributor.authorSancho, Juan V
dc.contributor.authorCortés, Miguel Ángel
dc.contributor.authorYusà, Vicent
dc.contributor.authorHernandez, Felix
dc.date.accessioned2012-10-03T11:54:42Z
dc.date.available2012-10-03T11:54:42Z
dc.date.issued2011
dc.identifier.citationFood Chemistry, 2011, May, Vol. 126, No. 2ca_CA
dc.identifier.issn0308-8146
dc.identifier.urihttp://hdl.handle.net/10234/48014
dc.description.abstractIn this work, a method has been developed for the ultrasensitive and selective determination of various regulated mycotoxins (aflatoxins G1, G2, B1, B2, M1, and ochratoxin A) in baby food commodities and milk, using ultra high pressure liquid chromatography (UHPLC) coupled to tandem mass spectrometry (MS/MS). The high sensitivity required for these compounds made necessary the application of a pre-concentration step based on solid phase extraction with immunoaffinity columns, after sample extraction with acetonitrile:water (80:20). Thanks to the fast high-resolution of UHPLC and the enhanced selectivity obtained with the triple quadrupole mass analyser in SRM mode, the chromatographic separation was achieved in only 4 min. Validation of the method was carried out in four different matrices (cereals infant formula, powdered milk for babies, milk with cereals for infants, and raw milk) by recovery experiments, using samples spiked at 0.025 and 0.1 μg kg−1 in quintuplicate. Satisfactory recoveries, between 80% and 110%, with RSDs lower than 15%, were obtained in all food matrices tested. Quantification was performed by external calibration with standards in solvent. The developed method has been applied to the analysis of samples collected from local markets. Positive findings were confirmed by acquiring two transitions (Q quantification, q confirmation) and evaluating their Q/q ratio.ca_CA
dc.language.isoengca_CA
dc.publisherElsevier
dc.rights© 2010 Elsevier Ltd. All rights reservedca_CA
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/*
dc.subjectAflatoxinsca_CA
dc.subjectBaby foodca_CA
dc.subjectOchratoxin Aca_CA
dc.subjectImmunoaffinity columnsca_CA
dc.subjectUHPLCca_CA
dc.subjectMS/MSca_CA
dc.titleUHPLC–MS/MS highly sensitive determination of aflatoxins, the aflatoxin metabolite M1 and ochratoxin A in baby food and milkca_CA
dc.typeinfo:eu-repo/semantics/articleca_CA
dc.identifier.doihttp://dx.doi.org/10.1016/j.foodchem.2010.11.056
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccessca_CA
dc.relation.publisherVersionhttp://www.sciencedirect.com/science/article/pii/S0308814610014743ca_CA


Ficheros en el ítem

FicherosTamañoFormatoVer

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem