Mostrar el registro sencillo del ítem
Detection and structural investigation of metabolites of stanozolol in human urine by liquid chromatography tandem mass spectrometry
dc.contributor.author | Pozo, Óscar J. | |
dc.contributor.author | Van Eenoo, Peter | |
dc.contributor.author | Deventer, Koen | |
dc.contributor.author | Lootens, Leens | |
dc.contributor.author | Grimalt Brea, Susana | |
dc.contributor.author | Sancho, Juan V | |
dc.contributor.author | Hernandez, Felix | |
dc.contributor.author | Meuleman, Philip | |
dc.contributor.author | Leroux-Roels, Geert | |
dc.contributor.author | Delbeke, Frans T. | |
dc.date.accessioned | 2012-08-07T09:56:06Z | |
dc.date.available | 2012-08-07T09:56:06Z | |
dc.date.issued | 2009 | |
dc.identifier | http://dx.doi.org/10.1016/j.steroids.2009.05.004 | |
dc.identifier.citation | Steroids, 74, , p. 837-852 | |
dc.identifier.issn | 0039128X | |
dc.identifier.uri | http://hdl.handle.net/10234/43757 | |
dc.description.abstract | The applicability of LC-MS/MS in precursor ion scan mode for the detection of urinary stanozolol metabolites has been studied. The product ion at m/z 81 has been selected as specific for stanozolol metabolites without a modification in A- or N-rings and the product ions at m/z 97 and 145 for the metabolites hydroxylated in the N-ring and 4-hydroxy-stanozolol metabolites, respectively. Under these conditions, the parent drug and up to 15 metabolites were found in a positive doping test sample. The study of a sample from a chimeric uPA-SCID mouse collected after the administration of stanozolol revealed the presence of 4 additional metabolites. The information obtained from the product ion spectra was used to develop a SRM method for the detection of 19 compounds. This SRM method was applied to several doping positive samples. All the metabolites were detected in both the uPA-SCID mouse sample and positive human samples and were not detected in none of the blank samples tested; confirming the metabolic nature of all the detected compounds. In addition, the application of the SRM method to a single human excretion study revealed that one of the metabolites (4ξ,16ξ-dihydroxy-stanozolol) could be detected in negative ionization mode for a longer period than those commonly used in the screening for stanozolol misuse (3′-hydroxy-stanozolol, 16β-hydroxy-stanozolol and 4β-hydroxy-stanozolol) in doping analysis. The application of the developed approach to several positive doping samples confirmed the usefulness of this metabolite for the screening of stanozolol misuse. Finally, a tentative structure for each detected metabolite has been proposed based on the product ion spectra measured with accurate masses using UPLC-QTOF MS. © 2009 Elsevier Inc. All rights reserved. | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.rights.uri | http://rightsstatements.org/vocab/CNE/1.0/ | * |
dc.subject | Anabolic steroids | |
dc.subject | Chimeric mice | |
dc.subject | Doping analysis | |
dc.subject | Metabolites | |
dc.subject | Precursor ion scan | |
dc.subject | QTOF MS | |
dc.title | Detection and structural investigation of metabolites of stanozolol in human urine by liquid chromatography tandem mass spectrometry | |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | http://dx.doi.org/10.1016/j.steroids.2009.05.004 | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
Ficheros en el ítem
Ficheros | Tamaño | Formato | Ver |
---|---|---|---|
No hay ficheros asociados a este ítem. |
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
QFA_Articles [819]
Articles de publicacions periòdiques