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dc.contributor.authorRuiz Ángel, M. J.
dc.contributor.authorTorres Lapasio, José Ramón
dc.contributor.authorGarcía Álvarez-Coque, M. C.
dc.contributor.authorCarda-Broch, Samuel
dc.date.accessioned2012-08-07T09:55:55Z
dc.date.available2012-08-07T09:55:55Z
dc.date.issued2009
dc.identifierhttp://dx.doi.org/10.1016/j.chroma.2009.02.016
dc.identifier.citationJournal of Chromatography A, 1216, 15, p. 3199-3209
dc.identifier.issn219673
dc.identifier.urihttp://hdl.handle.net/10234/43714
dc.description.abstractThe behaviour of a reversed-phase liquid chromatographic (RPLC) system (i.e. elution order, resolution and analysis time), used in the analysis of β-blockers with acetonitrile-water mobile phases, changes drastically upon addition of an anionic surfactant (sodium dodecyl sulphate, SDS). Surfactant monomers cover the alkyl-bonded phase in different extent depending on the concentration of both modifiers, in the ranges 1 × 10<sup>-3</sup>-0.15 M SDS and 5-50% acetonitrile. Meanwhile, the surfactant is dissolved in the mobile phase as free monomers, associated in small clusters or forming micelles. Four characteristic RPLC modes are yielded, with transition regions between them: hydro-organic, micellar, and low and high submicellar. The mobile phases in the two latter modes contain a concentration of SDS below or well above the critical micellar concentration (CMC) in water (i.e. 8 × 10<sup>-3</sup> M), and more than 30% acetonitrile. High submicellar RPLC appeared as the most promising mode, as it allowed full resolution of the β-blockers in practical times, while these were unresolved or highly retained in the other RPLC modes. The strong attraction of the cationic solutes to the anionic SDS makes a direct transfer mechanism between surfactant molecules in the stationary and mobile phases likely. © 2009 Elsevier B.V. All rights reserved.
dc.language.isoeng
dc.publisherElsevier
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/*
dc.subjectβ-Blockers
dc.subjectAcetonitrile
dc.subjectAnalysis time
dc.subjectDirect transfer mechanism
dc.subjectSelectivity
dc.subjectSodium dodecyl sulphate
dc.subjectSurfactant-mediated chromatographic systems
dc.titleSubmicellar and micellar reversed-phase liquid chromatographic modes applied to the separation of β-blockers
dc.typeinfo:eu-repo/semantics/article
dc.identifier.doihttp://dx.doi.org/10.1016/j.chroma.2009.02.016
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.type.versioninfo:eu-repo/semantics/publishedVersion


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