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Submicellar and micellar reversed-phase liquid chromatographic modes applied to the separation of β-blockers
dc.contributor.author | Ruiz Ángel, M. J. | |
dc.contributor.author | Torres Lapasio, José Ramón | |
dc.contributor.author | García Álvarez-Coque, M. C. | |
dc.contributor.author | Carda-Broch, Samuel | |
dc.date.accessioned | 2012-08-07T09:55:55Z | |
dc.date.available | 2012-08-07T09:55:55Z | |
dc.date.issued | 2009 | |
dc.identifier | http://dx.doi.org/10.1016/j.chroma.2009.02.016 | |
dc.identifier.citation | Journal of Chromatography A, 1216, 15, p. 3199-3209 | |
dc.identifier.issn | 219673 | |
dc.identifier.uri | http://hdl.handle.net/10234/43714 | |
dc.description.abstract | The behaviour of a reversed-phase liquid chromatographic (RPLC) system (i.e. elution order, resolution and analysis time), used in the analysis of β-blockers with acetonitrile-water mobile phases, changes drastically upon addition of an anionic surfactant (sodium dodecyl sulphate, SDS). Surfactant monomers cover the alkyl-bonded phase in different extent depending on the concentration of both modifiers, in the ranges 1 × 10<sup>-3</sup>-0.15 M SDS and 5-50% acetonitrile. Meanwhile, the surfactant is dissolved in the mobile phase as free monomers, associated in small clusters or forming micelles. Four characteristic RPLC modes are yielded, with transition regions between them: hydro-organic, micellar, and low and high submicellar. The mobile phases in the two latter modes contain a concentration of SDS below or well above the critical micellar concentration (CMC) in water (i.e. 8 × 10<sup>-3</sup> M), and more than 30% acetonitrile. High submicellar RPLC appeared as the most promising mode, as it allowed full resolution of the β-blockers in practical times, while these were unresolved or highly retained in the other RPLC modes. The strong attraction of the cationic solutes to the anionic SDS makes a direct transfer mechanism between surfactant molecules in the stationary and mobile phases likely. © 2009 Elsevier B.V. All rights reserved. | |
dc.language.iso | eng | |
dc.publisher | Elsevier | |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | * |
dc.subject | β-Blockers | |
dc.subject | Acetonitrile | |
dc.subject | Analysis time | |
dc.subject | Direct transfer mechanism | |
dc.subject | Selectivity | |
dc.subject | Sodium dodecyl sulphate | |
dc.subject | Surfactant-mediated chromatographic systems | |
dc.title | Submicellar and micellar reversed-phase liquid chromatographic modes applied to the separation of β-blockers | |
dc.type | info:eu-repo/semantics/article | |
dc.identifier.doi | http://dx.doi.org/10.1016/j.chroma.2009.02.016 | |
dc.rights.accessRights | info:eu-repo/semantics/restrictedAccess | |
dc.type.version | info:eu-repo/semantics/publishedVersion |
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