The Presence of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Transgenic Rapeseed Overexpressing a Trichoderma Thkel1 Gene Improves Plant Development and Yield
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Other documents of the author: Nicolás, Carlos; Calvo-Polanco, Monica; Poveda, Jorge; Alonso Ramírez, Ana; Ascaso-Pérez, Julio; Arbona, Vicent; Hermosa, Rosa
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comunitat-uji-handle3:10234/6999
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Title
The Presence of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Transgenic Rapeseed Overexpressing a Trichoderma Thkel1 Gene Improves Plant Development and YieldAuthor (s)
Date
2024-06Publisher
MDPIISSN
2077-0472Bibliographic citation
Nicolás, C.; Calvo-Polanco, M.; Poveda, J.; Alonso-Ramírez, A.; Ascaso, J.; Arbona, V.; Hermosa, R. The Presence of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Transgenic Rapeseed Overexpressing a Trichoderma Thkel1 Gene Improves Plant Development and Yield. Agriculture 2024, 14, 851. https://doi.org/10.3390/agriculture14060851Type
info:eu-repo/semantics/articlePublisher version
https://www.mdpi.com/2077-0472/14/6/851Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
Most of the plants belonging to the family of Brassicaceae are non-hosts for arbuscular mycorrhizal fungi (AMF). These plants are known to produce glucosinolates (GSL), a group of allelopathic compounds, with a role ... [+]
Most of the plants belonging to the family of Brassicaceae are non-hosts for arbuscular mycorrhizal fungi (AMF). These plants are known to produce glucosinolates (GSL), a group of allelopathic compounds, with a role in plant defense. The overexpression of the Thkel1 from Trichoderma harzianum in rapeseed (BnKel) plants, this gene encoding a protein that shares similarities with Brassicaceae plant’s nitrile-specifier and epithiospecifier proteins, modified GSL metabolism, reducing the accumulation of toxic isothiocyanates due to hydrolysis of these secondary metabolites. Here, we have analyzed the effect of AMF application on the GSL profiles and the development and yield of BnKel plants. Our results showed that the reduction of GSL compounds on transgenic plants was not enough to allow the formation of arbuscules and vesicles characteristics of an AMF mycorrhizal association. However, the inoculation of transgenic rapeseed plants expressing Thkel1 with AMF improved seed yield and fatty acid composition of the oilseed, showing a beneficial effect of AMF in these plants. The achievement of this effective beneficial association among mycorrhizas and rapeseed plants opens new opportunities in agribiotechnology for the use of AMF as biofertilizers in Brassicaceae crops with potential application in medical, animal and industrial biotechnology. [-]
Is part of
Agriculture, 2024, vol. 14, no 6Funder Name
European Regional Development Fund | Junta de Castilla y León | Ministerio de Ciencia e Innovación
Funder ID
http://dx.doi.org/10.13039/501100011033
Project code
SA230U13 | SA270P18 | SA094P20 | IR2020-1-USAL05 | LU-2018-04 | MCIN/PEICTI2021-2023/PDI2021-126575OB-I00
Project title or grant
Investigación de los mecanismos moleculares implicados en el aumento de la tolerancia de las plantas de trigo al estrés hídrico tras la aplicación de hongos beneficiosos.
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Except where otherwise noted, this item's license is described as Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).