Innovative Detection of Biomarkers Based on Chemiluminescent Nanoparticles and a Lensless Optical Sensor
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INVESTIGACIONMetadata
Title
Innovative Detection of Biomarkers Based on Chemiluminescent Nanoparticles and a Lensless Optical SensorAuthor (s)
Date
2024-04Publisher
MDPIISSN
2079-6374Bibliographic citation
Potrich, C.; Palmara, G.; Frascella, F.; Pancheri, L.; Lunelli, L. Innovative Detection of Biomarkers Based on Chemiluminescent Nanoparticles and a Lensless Optical Sensor. Biosensors 2024, 14, 184. https://doi.org/10.3390/bios14040184Type
info:eu-repo/semantics/articlePublisher version
https://www.mdpi.com/2079-6374/14/4/184Version
info:eu-repo/semantics/publishedVersionSubject
Abstract
The identification and quantification of biomarkers with innovative technologies is an urgent need for the precise diagnosis and follow up of human diseases. Body fluids offer a variety of informative biomarkers, which ... [+]
The identification and quantification of biomarkers with innovative technologies is an urgent need for the precise diagnosis and follow up of human diseases. Body fluids offer a variety of informative biomarkers, which are traditionally measured with time-consuming and expensive methods. In this context, lateral flow tests (LFTs) represent a rapid and low-cost technology with a sensitivity that is potentially improvable by chemiluminescence biosensing. Here, an LFT based on gold nanoparticles functionalized with antibodies labeled with the enzyme horseradish peroxidase is combined with a lensless biosensor. This biosensor comprises four Silicon Photomultipliers (SiPM) coupled in close proximity to the LFT strip. Microfluidics for liquid handling complete the system. The development and the setup of the biosensor is carefully described and characterized. C-reactive protein was selected as a proof-of-concept biomarker to define the limit of detection, which resulted in about 0.8 pM when gold nanoparticles were used. The rapid readout (less than 5 min) and the absence of sample preparation make this biosensor promising for the direct and fast detection of human biomarkers. [-]
Is part of
Biosensors, 2024, vol. 14, no 4Related data
https://www.mdpi.com/article/10.3390/bios14040184/s1Funder Name
VRT (Fondazione Valorizzazione Ricerca Trentina)
Project code
SiPoBio
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info:eu-repo/semantics/openAccess
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- INAM_Articles [527]
Except where otherwise noted, this item's license is described as Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).