Innovative Detection of Biomarkers Based on Chemiluminescent Nanoparticles and a Lensless Optical Sensor
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INVESTIGACIONMetadades
Títol
Innovative Detection of Biomarkers Based on Chemiluminescent Nanoparticles and a Lensless Optical SensorAutoria
Data de publicació
2024-04Editor
MDPIISSN
2079-6374Cita bibliogràfica
Potrich, C.; Palmara, G.; Frascella, F.; Pancheri, L.; Lunelli, L. Innovative Detection of Biomarkers Based on Chemiluminescent Nanoparticles and a Lensless Optical Sensor. Biosensors 2024, 14, 184. https://doi.org/10.3390/bios14040184Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
https://www.mdpi.com/2079-6374/14/4/184Versió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
The identification and quantification of biomarkers with innovative technologies is an urgent need for the precise diagnosis and follow up of human diseases. Body fluids offer a variety of informative biomarkers, which ... [+]
The identification and quantification of biomarkers with innovative technologies is an urgent need for the precise diagnosis and follow up of human diseases. Body fluids offer a variety of informative biomarkers, which are traditionally measured with time-consuming and expensive methods. In this context, lateral flow tests (LFTs) represent a rapid and low-cost technology with a sensitivity that is potentially improvable by chemiluminescence biosensing. Here, an LFT based on gold nanoparticles functionalized with antibodies labeled with the enzyme horseradish peroxidase is combined with a lensless biosensor. This biosensor comprises four Silicon Photomultipliers (SiPM) coupled in close proximity to the LFT strip. Microfluidics for liquid handling complete the system. The development and the setup of the biosensor is carefully described and characterized. C-reactive protein was selected as a proof-of-concept biomarker to define the limit of detection, which resulted in about 0.8 pM when gold nanoparticles were used. The rapid readout (less than 5 min) and the absence of sample preparation make this biosensor promising for the direct and fast detection of human biomarkers. [-]
Publicat a
Biosensors, 2024, vol. 14, no 4Dades relacionades
https://www.mdpi.com/article/10.3390/bios14040184/s1Entitat finançadora
VRT (Fondazione Valorizzazione Ricerca Trentina)
Codi del projecte o subvenció
SiPoBio
Drets d'accés
info:eu-repo/semantics/openAccess
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Except where otherwise noted, this item's license is described as Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).