Adjusting and validating a procedure for parenteral anaesthesia in neonatal mice
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INVESTIGACIONMetadades
Títol
Adjusting and validating a procedure for parenteral anaesthesia in neonatal miceAutoria
Data de publicació
2024-03-18Editor
Sage PublilcationsCita bibliogràfica
Goterris-Cerisuelo R, Sanahuja-Irene S, Sánchez-Catalán MJ, Martínez-García F. Adjusting and validating a procedure for parenteral anaesthesia in neonatal mice. Laboratory Animals. 2024;0(0). doi:10.1177/00236772231219821Tipus de document
info:eu-repo/semantics/articleVersió de l'editorial
https://journals.sagepub.com/doi/abs/10.1177/00236772231219821Versió
info:eu-repo/semantics/acceptedVersionParaules clau / Matèries
Resum
For neonatal pups, parenteral anaesthesia is said to be not reliable as low doses induce no anaesthesia whereas high doses render high mortality rates. In this work we have adapted parenteral anaesthesia procedures ... [+]
For neonatal pups, parenteral anaesthesia is said to be not reliable as low doses induce no anaesthesia whereas high doses render high mortality rates. In this work we have adapted parenteral anaesthesia procedures approved for pups >7 days of age, to anaesthetize neonatal animals (postnatal days 3–4; P3–P4) for keeping them immobile for a long period. In our first experiment we analysed the behaviour of P3–P4 mouse pups for 70 min after intraperitoneal administration of low (37.5/3.75 mg/kg) or high (50/5) doses of a ketamine/xylazine anaesthetic mixture, both in the low range as compared with dosages employed in adults. Pups became immobile in ≈7 min and remained immobile for ≈45 min, irrespective of the age and dose of anaesthesia, younger pups (P3) being apparently more sensitive to the dosage. In the second experiment, we studied the response of P3 pups to mildly nociceptive stimulations, performed with a 4.0 g von Frey filament applied to the dorsal aspect of their paws. These stimuli elicited reaction in 100% of the cases in non-anaesthetized pups. The results indicate that the high dose significantly reduced responses as compared with the low dose of anaesthesia. With the low dose, <40% of the pups were unresponsive to nociceptive stimulation, whereas the high dose resulted in 50–60% of the animals not responding. Mortality was low irrespective of age or dose, suggesting that doses can be further increased if needed for invasive experimental procedures. [-]
Publicat a
Laboratory Animals, 2024.Entitat finançadora
Ministerio de Ciencia e Innovación | Universitat Jaume I
Codi del projecte o subvenció
PID2019-107322GB-C21 | UJI-A2019-14
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