The rostromedial tegmental nucleus RMTg is not a critical site for ethanol-induced motor activation in rats
Impacto
Scholar |
Otros documentos de la autoría: Esposito Zapero, Claudia; Fernández Rodríguez, Sandra; Sánchez-Catalán, María José; Zornoza, Teodoro; Cano-Cebrián, Maria José; Granero, Luis
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
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INVESTIGACIONMetadatos
Título
The rostromedial tegmental nucleus RMTg is not a critical site for ethanol-induced motor activation in ratsAutoría
Fecha de publicación
2023-07-20Editor
Springer NatureISSN
0033-3158; 1432-2072Cita bibliográfica
Esposito-Zapero, C., Fernández-Rodríguez, S., Sánchez-Catalán, M.J. et al. The rostromedial tegmental nucleus RMTg is not a critical site for ethanol-induced motor activation in rats. Psychopharmacology 240, 2071–2080 (2023).Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
-Rationale :
Opioid drugs indirectly activate dopamine (DA) neurons in the ventral tegmental area (VTA) through a disinhibition mechanism mediated by mu opioid receptors (MORs) present both on the GABA projection ... [+]
-Rationale :
Opioid drugs indirectly activate dopamine (DA) neurons in the ventral tegmental area (VTA) through a disinhibition mechanism mediated by mu opioid receptors (MORs) present both on the GABA projection neurons located in the medial tegmental nucleus/tail of the VTA (RMTg/tVTA) and on the VTA GABA interneurons. It is well demonstrated that ethanol, like opioid drugs, provokes VTA DA neuron disinhibition by interacting (through its secondary metabolite, salsolinol) with MORs present in VTA GABA interneurons, but it is not known whether ethanol could disinhibit VTA DA neurons through the MORs present in the RMTg/tVTA.
-Objectives :
The objective of the present study was to determine whether ethanol, directly microinjected into the tVTA/RMTg, is also able to induce VTA DA neurons disinhibition.
-Methods :
Disinhibition of VTA DA neurons was indirectly assessed through the analysis of the motor activity of rats. Cannulae were placed into the tVTA/RMTg to perform microinjections of DAMGO (0.13 nmol), ethanol (150 or 300 nmol) or acetaldehyde (250 nmol) in animals pre-treated with either aCSF or the irreversible antagonist of MORs, beta-funaltrexamine (beta-FNA; 2.5 nmol). After injections, spontaneous activity was monitored for 30 min.
-Results :
Neither ethanol nor acetaldehyde directly administered into the RMTg/tVTA were able to increase the locomotor activity of rats at doses that, in previous studies performed in the posterior VTA, were effective in increasing motor activities. However, microinjections of 0.13 nmol of DAMGO into the tVTA/RMTg significantly increased the locomotor activity of rats. These activating effects were reduced by local pre-treatment of rats with beta-FNA (2.5 nmol).
-Conclusions :
The tVTA/RMTg does not appear to be a key brain region for the disinhibiting action of ethanol on VTA DA neurons. The absence of dopamine in the tVTA/RMTg extracellular medium, the lack of local ethanol metabolism or both could explain the present results. [-]
Publicado en
Psychopharmacology Vol. 240 (2023)Entidad financiadora
CRUE-CSIC | Generalitat Valenciana. Conselleria de Educación, Investigación, Cultura y Deporte
Código del proyecto o subvención
GVA2016-096 | GVA2019-116
Derechos de acceso
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- MED_Articles [657]