Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content
Impacto
Scholar |
Otros documentos de la autoría: Vazquez-Vilar, Marta; Fernández-del-Carmen, Asun; Garcia-Carpintero, Victor; Drapal, Margit; Presa, Silvia; Ricci, Dorotea; Diretto, Gianfranco; Rambla, José L.; Fernandez-Muñoz, Rafael; Espinosa-Ruiz, Ana; Fraser, Paul; Martin, Cathie; Granell, Antonio; ORZAEZ, DIEGO
Metadatos
Mostrar el registro completo del ítemcomunitat-uji-handle:10234/9
comunitat-uji-handle2:10234/197672
comunitat-uji-handle3:10234/197673
comunitat-uji-handle4:
INVESTIGACIONMetadatos
Título
Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids contentAutoría
Fecha de publicación
2023-09-25Editor
Wiley; Association of Applied Biologists; Society for Experimental BiologyISSN
1467-7644; 1467-7652Cita bibliográfica
Vazquez-Vilar, M., Fernandez-del-Carmen, A., Garcia-Carpintero, V., Drapal, M., Presa, S., Ricci, D., Diretto, G., Rambla, J.L., Fernandez-Muñoz, R., Espinosa-Ruiz, A., Fraser, P.D., Martin, C., Granell, A. and Orzaez, D. (2023), Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content. Plant Biotechnol. J, 21: 2683-2697.Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Higher dietary intakes of flavonoids may have a beneficial role in cardiovascular disease prevention. Additionally, supplementation of branched-chain amino acids (BCAAs) in vegan diets can reduce risks associated to ... [+]
Higher dietary intakes of flavonoids may have a beneficial role in cardiovascular disease prevention. Additionally, supplementation of branched-chain amino acids (BCAAs) in vegan diets can reduce risks associated to their deficiency, particularly in older adults, which can cause loss of skeletal muscle strength and mass. Most plant-derived foods contain only small amounts of BCAAs, and those plants with high levels of flavonoids are not eaten broadly. Here we describe the generation of metabolically engineered cisgenic tomatoes enriched in both flavonoids and BCAAs. In this approach, coding and regulatory DNA elements, all derived from the tomato genome, were combined to obtain a herbicide-resistant version of an acetolactate synthase (mSlALS) gene expressed broadly and a MYB12-like transcription factor (SlMYB12) expressed in a fruit-specific manner. The mSlALS played a dual role, as a selectable marker as well as being key enzyme in BCAA enrichment. The resulting cisgenic tomatoes were highly enriched in Leucine (21-fold compared to wild-type levels), Valine (ninefold) and Isoleucine (threefold) and concomitantly biofortified in several antioxidant flavonoids including kaempferol (64-fold) and quercetin (45-fold). Comprehensive metabolomic and transcriptomic analysis of the biofortified cisgenic tomatoes revealed marked differences to wild type and could serve to evaluate the safety of these biofortified fruits for human consumption. [-]
Publicado en
Plant Biotechnology Journal, Vol. 21, Issue12 (December 2023)Entidad financiadora
Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación (AEI)
Código del proyecto o subvención
PID2019-108203RB-100
Derechos de acceso
info:eu-repo/semantics/openAccess
Aparece en las colecciones
- BBICN_Articles [90]