Detection of SARS-CoV-2 in aerosols in long term care facilities and other indoor spaces with known COVID-19 outbreaks
Impacto
Scholar |
Otros documentos de la autoría: Barberá-Riera, María; Barneo-Muñoz, Manuela; Gascó Laborda, Juan Carlos; Bellido-Blasco, Juan Bautista; Porru, Simona; Alfaro Pérez, Carlos; Esteve-Cano, Vicente; Carrasco Espí, Paula; Rebagliato, Marisa; de Llanos Frutos, Rosa; Delgado-Saborit, Juana Maria
Metadatos
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INVESTIGACIONMetadatos
Título
Detection of SARS-CoV-2 in aerosols in long term care facilities and other indoor spaces with known COVID-19 outbreaksAutoría
Fecha de publicación
2023-11-23Editor
ElsevierCita bibliográfica
BARBERÁ-RIERA, M., et al. Detection of SARS-CoV-2 in aerosols in long term care facilities and other indoor spaces with known COVID-19 outbreaks. Environmental Research, 2024, vol. 242, p. 117730.Tipo de documento
info:eu-repo/semantics/articleVersión
info:eu-repo/semantics/acceptedVersionPalabras clave / Materias
Resumen
Coronavirus outbreaks are likely to occur in crowded and congregate indoor spaces, and their effects are most severe in vulnerable long term care facilities (LTCFs) residents. Public health officers benefit from tools ... [+]
Coronavirus outbreaks are likely to occur in crowded and congregate indoor spaces, and their effects are most severe in vulnerable long term care facilities (LTCFs) residents. Public health officers benefit from tools that allow them to control COVID-19 outbreaks in vulnerable settings such as LTCFs, but which could be translated in the future to control other known and future virus outbreaks. This study aims to develop and test a methodology based on detection of SARS-CoV-2 in aerosol samples collected with personal pumps that could be easily implemented by public health officers. The proposed methodology was used to investigate the levels of SARSCoV-2 in aerosol in indoor settings, mainly focusing on LTCFs, suffering COVID-19 outbreaks, or in the presence of known COVID-19 cases, and targeting the initial days after diagnosis. Aerosol samples (N = 18) were collected between November 2020 and March 2022 in Castell ' o (Spain) from LTCFs, merchant ships and a private home with recently infected COVID-19 cases. Sampling was performed for 24-h, onto 47 mm polytetrafluoroethylene (PTFE) and quartz filters, connected to personal pumps at 2 and 4 L/ min respectively. RNA from filters was extracted and SARS-CoV-2 was determined by detection of regions N1 and N2 of the nucleocapsid gene alongside the E gene using RT-PCR technique. SARS-CoV-2 genetic material was detected in 87.5% samples. Concentrations ranged ND-19,525 gc/m3 (gene E). No genetic traces were detected in rooms from contacts that were isolated as a preventative measure. Very high levels were also measured at locations with poor ventilation. Aerosol measurement conducted with the proposed methodology provided useful information to public health officers and contributed to manage and control 12 different COVID-19 outbreaks. SARS-CoV-2 was detected in aerosol samples collected during outbreaks in congregate spaces. Indoor aerosol sampling is a useful tool in the early detection and management of COVID-19 outbreaks and supports epidemiological investigations. [-]
Entidad financiadora
Fundación BBVA | Generalitat Valenciana | Ministerio de Educación y Formación Profesional, Spanish Government | European Union-Next Generation EU | Universitat Jaume I
Código del proyecto o subvención
ECO-VET-COVID-BBVA_0029 | CIDEGENT/2019/064 | BGP18/00062 | MGS/2021/19(UP2021-021) | POSDOC-A/2018/35 | PREDOC/2021/07
Título del proyecto o subvención
Detección del SARS-CoV-2 en aerosol atmosférico como herramienta de vigilancia y alerta de la transmisión del COVID-19– AYUDAS FUNDACIÓN BBVA A EQUIPOS DE INVESTIGACIÓN CIENTÍFICA SARS-CoV-2 y COVID-19 | Detección del SARS-CoV-2 en aerosol atmosférico en ambientes interiores como herramienta de evaluación de medidas sanitarias de prevención y de alerta de la transmisión del COVID-19 (IDIEX COVID-proyecto 4)
Derechos de acceso
© 2023 Elsevier Inc. All rights reserved.
info:eu-repo/semantics/embargoedAccess
info:eu-repo/semantics/embargoedAccess
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