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Biocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystals
dc.contributor.author | Alves da Silva Pimentel, Bruna Natalia | |
dc.contributor.author | De Annunzio, Sarah Raquel | |
dc.contributor.author | Assis, Marcelo de | |
dc.contributor.author | Barbugli, Paula | |
dc.contributor.author | Longo, Elson | |
dc.contributor.author | Vergani, Carlos Eduardo | |
dc.date.accessioned | 2023-10-16T17:18:24Z | |
dc.date.available | 2023-10-16T17:18:24Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Pimentel BNAS, De Annunzio SR, Assis M, Barbugli PA, Longo E and Vergani CE (2023), Biocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystals. Front. Bioeng. Biotechnol. 11:1215438. doi: 10.3389/fbioe.2023.1215438 | ca_CA |
dc.identifier.issn | 2296-4185 | |
dc.identifier.uri | http://hdl.handle.net/10234/204510 | |
dc.description.abstract | Silver tungstate (α-Ag2WO4), silver molybdate (β-Ag2MoO4), and silver vanadate (α-AgVO3) microcrystals have shown interesting antimicrobial properties. However, their biocompatibility is not yet fully understood. Cytotoxicity and the inflammatory response of silver-containing microcrystals were analyzed in THP-1 and THP-1 differentiated as macrophage-like cells, with the alamarBlue™ assay, flow cytometry, confocal microscopy, and ELISA. The present investigation also evaluated redox signaling and the production of cytokines (TNFα, IL-1β, IL-6, and IL-8) and matrix metalloproteinases (MMP-8 and -9). The results showed that α-AgVO3 (3.9 μg/mL) did not affect cell viability (p > 0.05). α-Ag2WO4 (7.81 μg/mL), β-Ag2MoO4 (15.62 μg/mL), and α-AgVO3 (15.62 μg/mL) slightly decreased cell viability (p ≤ 0.003). All silver-containing microcrystals induced the production of O2 − and this effect was mitigated by Reactive Oxygen Species (ROS) scavenger and N-acetylcysteine (NAC). TNFα, IL-6 and IL-1β were not detected in THP-1 cells, while their production was either lower (p ≤ 0.0321) or similar to the control group (p ≥ 0.1048) for macrophage-like cells. The production of IL-8 by both cellular phenotypes was similar to the control group (p ≥ 0.3570). The release of MMP-8 was not detected in any condition in THP-1 cells. Although MMP-9 was released by THP-1 cells exposed to α-AgVO3 (3.9 μg/mL), no significant difference was found with control (p = 0.7). Regarding macrophage-like cells, the release of MMP-8 and -9 decreased in the presence of all microcrystals (p ≤ 0.010). Overall, the present work shows a promising biocompatibility profile of, α-Ag2WO4, βAg2MoO4, and α-AgVO3 microcrystals. | ca_CA |
dc.format.extent | 13 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.relation.isPartOf | Frontiers in Bioengineering and Biotechnology 11:1215438 | ca_CA |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | ca_CA |
dc.subject | silver-based metal oxides | ca_CA |
dc.subject | cytokines | ca_CA |
dc.subject | matrix metalloproteinases | ca_CA |
dc.subject | reactive oxygen species | ca_CA |
dc.subject | monocytes | ca_CA |
dc.subject | macrophages | ca_CA |
dc.title | Biocompatibility and inflammatory response of silver tungstate, silver molybdate, and silver vanadate microcrystals | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.3389/fbioe.2023.1215438 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
project.funder.name | São Paulo Research Foundation (FAPESP) | ca_CA |
project.funder.name | FAPESP | ca_CA |
project.funder.name | European Union-Next Generation | ca_CA |
oaire.awardNumber | 2013/07296-2 (CDMF) | ca_CA |
oaire.awardNumber | 2018/01677-8 | ca_CA |
oaire.awardNumber | 2022/08487-5 | ca_CA |
oaire.awardNumber | MGS/2021/21 (UP 2021-021) | ca_CA |
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