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Proteomic Analysis of Mesenchymal Stem Cells and Monocyte Co-Cultures Exposed to a Bioactive Silica-Based Sol–Gel Coating
dc.contributor.author | Cerqueira, Andreia | |
dc.contributor.author | Romero-Gavilán, Francisco J | |
dc.contributor.author | Helmholz, Heike | |
dc.contributor.author | Azkargorta, Mikel | |
dc.contributor.author | Elortza, Félix | |
dc.contributor.author | Gurruchaga, Mariló | |
dc.contributor.author | Goñi, Isabel | |
dc.contributor.author | Willumeit-Römer, Regine | |
dc.contributor.author | Suay, Julio | |
dc.date.accessioned | 2023-06-09T07:22:10Z | |
dc.date.available | 2023-06-09T07:22:10Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Cerqueira, A.; Romero-Gavilán, F.; Helmholz, H.; Azkargorta, M; Elortza, F.; Gurruchaga, M.; Goñi, I.; Willumeit-Römer, R.: Suay. J. Proteomic Analysis of Mesenchymal Stem Cells and Monocyte Co-Cultures Exposed to a Bioactive Silica-Based Sol–Gel Coating. ACS Biomater. Sci. Eng. 2023. DOI: 10.1021/acsbiomaterials.3c00254 | ca_CA |
dc.identifier.issn | 2373-9878 | |
dc.identifier.uri | http://hdl.handle.net/10234/202766 | |
dc.description.abstract | New methodologies capable of extensively analyzing the cell-material interactions are necessary to improve current in vitro characterization methods, and proteomics is a viable alternative. Also, many studies are focused on monocultures, even though co-cultures model better the natural tissue. For instance, human mesenchymal stem cells (MSCs) modulate immune responses and promote bone repair through interaction with other cell types. Here, label-free liquid chromatography tandem mass spectroscopy proteomic methods were applied for the first time to characterize HUCPV (MSC) and CD14+ monocytes co-cultures exposed to a bioactive sol–gel coating (MT). PANTHER, DAVID, and STRING were employed for data integration. Fluorescence microscopy, enzyme-linked immunosorbent assay, and ALP activity were measured for further characterization. Regarding the HUCPV response, MT mainly affected cell adhesion by decreasing integrins, RHOC, and CAD13 expression. In contrast, MT augmented CD14+ cell areas and integrins, Rho family GTPases, actins, myosins, and 14-3-3 expression. Also, anti-inflammatory (APOE, LEG9, LEG3, and LEG1) and antioxidant (peroxiredoxins, GSTO1, GPX1, GSHR, CATA, and SODM) proteins were overexpressed. On co-cultures, collagens (CO5A1, CO3A1, CO6A1, CO6A2, CO1A2, CO1A1, and CO6A3), cell adhesion, and pro-inflammatory proteins were downregulated. Thus, cell adhesion appears to be mainly regulated by the material, while inflammation is impacted by both cellular cross-talk and the material. Altogether, we conclude that applied proteomic approaches show its potential in biomaterial characterization, even in complex systems. | ca_CA |
dc.description.sponsorShip | Funding for open access charge: CRUE-Universitat Jaume I | |
dc.format.extent | 14 p. | ca_CA |
dc.format.mimetype | application/pdf | ca_CA |
dc.language.iso | eng | ca_CA |
dc.publisher | American Chemical Society | ca_CA |
dc.relation | Desarrollo de implantes dentales con popiedades osteogenicas para la universalizacion de receptores. Determinacion de patrones de proteinas de la eficacia regenerativa | ca_CA |
dc.relation.isPartOf | ACS Biomaterials Science and Engineering, 2023 | ca_CA |
dc.relation.uri | https://figshare.com/collections/Proteomic_Analysis_of_Mesenchymal_Stem_Cells_and_Monocyte_Co-Cultures_Exposed_to_a_Bioactive_Silica-Based_Sol_Gel_Coating/6655041 | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | ca_CA |
dc.subject | biomaterials | ca_CA |
dc.subject | osteoimmunology | ca_CA |
dc.subject | co-cultures | ca_CA |
dc.subject | proteomics | ca_CA |
dc.subject | sol−gel coatings | ca_CA |
dc.title | Proteomic Analysis of Mesenchymal Stem Cells and Monocyte Co-Cultures Exposed to a Bioactive Silica-Based Sol–Gel Coating | ca_CA |
dc.type | info:eu-repo/semantics/article | ca_CA |
dc.identifier.doi | https://doi.org/10.1021/acsbiomaterials.3c00254 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | ca_CA |
dc.relation.publisherVersion | https://pubs.acs.org/doi/full/10.1021/acsbiomaterials.3c00254 | ca_CA |
dc.description.sponsorship | This work was supported by MINECO [MAT2017-86043-R; RTC-2017-6147-1], Generalitat Valenciana [GRISOLIAP/2018/091, BEFPI/2021/043, PROMETEO/2020/069], Universitat Jaume I [UJI-B2017-37], and the University of the Basque Country [GIU18/189]. Andreia Cerqueira was supported by the Margarita Salas postdoctoral contract MGS/2022/10 (UP2022-024) financed by the European Union-NextGenerationEU. The University Medical Centre Hamburg-Eppendorf (Hamburg, Germany) and the Clinics for Gynecology AGAPLESION BETHESDA Hospital provided the blood and tissue for cell isolation. The authors would like to thank Raquel Oliver, Jose Ortega, Iraide Escobés, and Anke Borkam-Schuster for their valuable technical assistance and Antonio Coso (GMI-Ilerimplant) for producing the titanium discs. | |
dc.type.version | info:eu-repo/semantics/publishedVersion | ca_CA |
project.funder.identifier | http://dx.doi.org/10.13039/501100011033 | ca_CA |
project.funder.name | Ministerio de Economía y Competitividad (España) | ca_CA |
project.funder.name | Generalitat Valenciana | ca_CA |
project.funder.name | Universitat Jaume I | ca_CA |
project.funder.name | Universidad del País Vasco/Euskal Herriko Unibertsitatea | ca_CA |
project.funder.name | European Union-NextGenerationEU | ca_CA |
oaire.awardNumber | MINECO/ICTI2013-2016/MAT2017-86043-R | ca_CA |
oaire.awardNumber | MINECO//RTC-2017-6147-1 | ca_CA |
oaire.awardNumber | GRISOLIAP/2018/091 | ca_CA |
oaire.awardNumber | BEFPI/2021/043 | ca_CA |
oaire.awardNumber | PROMETEO/2020/069 | ca_CA |
oaire.awardNumber | UJI-B2017-37 | ca_CA |
oaire.awardNumber | GIU18/189 | ca_CA |
oaire.awardNumber | UP2022-024 | ca_CA |
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