Electrical Stimulation Increases Axonal Growth from Dorsal Root Ganglia Co-Cultured with Schwann Cells in Highly Aligned PLA-PPy-Au Microfiber Substrates
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Título
Electrical Stimulation Increases Axonal Growth from Dorsal Root Ganglia Co-Cultured with Schwann Cells in Highly Aligned PLA-PPy-Au Microfiber SubstratesAutoría
Fecha de publicación
2022-06-07Editor
MDPICita bibliográfica
Gisbert Roca, F.; Serrano Requena, S.; Monleón Pradas, M.; Martínez-Ramos, C. Electrical Stimulation Increases Axonal Growth from Dorsal Root Ganglia Co-Cultured with Schwann Cells in Highly Aligned PLA-PPy-Au Microfiber Substrates. Int. J. Mol. Sci. 2022, 23, 6362. https://doi.org/ 10.3390/ijms23126362Tipo de documento
info:eu-repo/semantics/articleVersión de la editorial
https://www.mdpi.com/1422-0067/23/12/6362Versión
info:eu-repo/semantics/publishedVersionPalabras clave / Materias
Resumen
Nerve regeneration is a slow process that needs to be guided for distances greater than
5 mm. For this reason, different strategies are being studied to guide axonal growth and accelerate
the axonal growth rate. In ... [+]
Nerve regeneration is a slow process that needs to be guided for distances greater than
5 mm. For this reason, different strategies are being studied to guide axonal growth and accelerate
the axonal growth rate. In this study, we employ an electroconductive fibrillar substrate that is able
to topographically guide axonal growth while accelerating the axonal growth rate when subjected
to an exogenous electric field. Dorsal root ganglia were seeded in co-culture with Schwann cells on
a substrate of polylactic acid microfibers coated with the electroconductive polymer polypyrrole,
adding gold microfibers to increase its electrical conductivity. The substrate is capable of guiding
axonal growth in a highly aligned manner and, when subjected to an electrical stimulation, an
improvement in axonal growth is observed. As a result, an increase in the maximum length of the
axons of 19.2% and an increase in the area occupied by the axons of 40% were obtained. In addition,
an upregulation of the genes related to axon guidance, axogenesis, Schwann cells, proliferation and
neurotrophins was observed for the electrically stimulated group. Therefore, our device is a good
candidate for nerve regeneration therapies. [-]
Publicado en
Int. J. Mol. Sci. 2022, 23(12), 6362Entidad financiadora
Ministerio de Ciencia, Innovación y Universidades (Spain) | H2020 Program
Código del proyecto o subvención
RTI2018-095872-B-C22/ERDF | RISEUP 964562
Derechos de acceso
© 2022 by the authors
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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