Impact of Ageing on Female Metabolic Flexibility: A Cross-Sectional Pilot Study in over-60 Active Women

Abstract

Background: Ageing affects metabolic flexibility, although physical status could influence this relationship. This cross-sectional study aims to describe and analyse the metabolic flexibility/inflexibility in a group of active older women, together with the impact of ageing and physical status on their oxidation rates and maximal fat oxidation (MFO). Methods: Fifteen volunteers (69.00 ± 6.97 years)—from 24 women—completed an incremental cycling test until the second ventilatory threshold. Intensity increased 10 W each 3 min 15 s, starting at 30 W. Gas exchange, heart rate, rate of perceived effort, pain scale and muscle power were registered, together with lactate. VO2 and VCO2 were considered for fat and carbohydrate oxidation (FATox and CHOox; Frayn’s equation) at intensities 60%, 80% and 100% from the peak power in the test (P100). Psychophysiological parameters were compared at MFO/FATmax and P100, together with the energy expenditure calculations around MFO (included FAT and CHO contributions), and the main correlation analyses, with and without P100 and VO2 as covariates. Results: FATox was low at MFO (0.13; 95% CI [0.09–0.17] mg/min/kgFFM; 3.50; 95% CI [2.49–4.50] mg/min/kgFFM), with short oxidation-rate curves shifting down and leftward. CHOox and FATox were both low for reduced power with age (77.14 ± 18.58 W and 39.29 ± 9.17 W at P100 and MFO, respectively), all accompanied by a fall in energy expenditure (5.44 ± 2.58 kcal/min and 3.32 ± 1.55 kcal/min at P100 and MFO, respectively). Power appears as a determinant factor, given its strong and negative significant association with age (r = − 0.85, p < 0.005; R2 = 0.72) and moderate with MFO (r = − 0.54, p = 0.04; R2 = 0.29). In turn, energy expenditure shows a positive and moderate association with muscle power (r = 52, p = 0.04). Conclusions: Despite the drop in substrates oxidation with age, physical status (i.e. larger muscular power and energy expenditure) suggests a key role in the preservation of metabolic health with ageing in active women.