Photodynamic inactivation of Staphylococcus aureus in the presence of aggregation-prone photosensitizers based on BODIPY used at submicromolar concentrations
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INVESTIGACIONMetadades
Títol
Photodynamic inactivation of Staphylococcus aureus in the presence of aggregation-prone photosensitizers based on BODIPY used at submicromolar concentrationsData de publicació
2022-08-10Editor
Elsevier B.V.ISSN
1011-1344Cita bibliogràfica
Navarro-Barreda, D., de Llanos, R., Miravet, J. F., & Galindo, F. (2022). Photodynamic inactivation of Staphylococcus aureus in the presence of aggregation-prone photosensitizers based on BODIPY used at submicromolar concentrations. Journal of Photochemistry and Photobiology B: Biology, 235, 112543Tipus de document
info:eu-repo/semantics/articleVersió
info:eu-repo/semantics/publishedVersionParaules clau / Matèries
Resum
Two new brominated BODIPYs (1 and 2) bearing amino acid-based chains (l-valine for 1, and dimethyl-l-lysine for 2) were synthesized and characterized. In organic solvents, 1 and 2 were fully soluble and showed the ... [+]
Two new brominated BODIPYs (1 and 2) bearing amino acid-based chains (l-valine for 1, and dimethyl-l-lysine for 2) were synthesized and characterized. In organic solvents, 1 and 2 were fully soluble and showed the photophysical properties expected for brominated BODIPY dyes, including efficient generation of singlet oxygen (1O2), upon irradiation. In contrast, in aqueous media, both compounds were prone to aggregation and the photo-induced generation of 1O2 was halted. Despite the lack of generation of this reactive species in aqueous media (in cuvette), both 1 and 2 have positive antimicrobial Photodynamic Inactivation (aPDI) effect. The activity against gram-positive Staphylococcus aureus and gram-negative Escherichia coli was determined through the inactivation curves, with a total energy dose of 5.3 J/cm2 (white light LED used as an energy source). Compound 2 was highly active against both gram-positive and gram-negative bacteria (3 log CFU/mL reduction was obtained at 0.16 μM for S. aureus and 2.5–5.0 μM for E. coli), whereas 1 was less effective to kill S. aureus (3 log CFU/mL at 0.32 μM) and ineffective for E. coli. The higher efficiency of 2, as compared to 1, to reduce the population of bacteria, can reside in the presence of a protonatable residue in 2, allowing a more effective interaction of this molecule with the cell walls of the microorganisms. In order to explain the lack of reactivity in pure aqueous media (in cuvette) and the contrasting good activity in the presence of bacterial cells it can be hypothesized that upon interaction with the walls of the microorganisms, the aggregated photosensitizers suffer a disaggregation process restoring the ability to generate 1O2, and hence leading to efficient photodynamic activity against these pathogenic microorganisms, in agreement with the similar effect observed recently for porphyrinoid photosensitizers. [-]
Publicat a
Journal of Photochemistry and Photobiology B: Biology, Vol. 235 (October 2022)Entitat finançadora
Ministerio de Ciencia e Innovación | European Regional Development Fund of the European Union | Universitat Jaume I | Ministerio de Educación y Formación Profesional
Codi del projecte o subvenció
RTI2018-101675-BI00 | UJI-B2021-51 | UJI-A2020- 15 | BGP18/00062
Drets d'accés
© 2022 The Authors
info:eu-repo/semantics/openAccess
info:eu-repo/semantics/openAccess
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